Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1993 Aug 25;21(17):4133-41.
doi: 10.1093/nar/21.17.4133.

Mutational analysis of the Saccharomyces cerevisiae general regulatory factor CP1

D C Masison  1 , K F O'Connell, R E Baker
Affiliations
Free PMC article

Mutational analysis of the Saccharomyces cerevisiae general regulatory factor CP1

D C Masison et al. Nucleic Acids Res. .
Free PMC article

Abstract

The Saccharomyces cerevisiae general regulatory factor CP1, a helix-loop-helix protein that binds the centromere DNA element I (CDEI) of yeast centromeres, is required in yeast for optimal centromere function and for methionine prototrophy. Mutant alleles of CEP1, the gene encoding CP1, were generated by linker insertion, 5'- and 3'-deletion, and random mutagenesis and assayed for DNA binding activity and their ability to confer CP1 function when expressed in yeast. A heterologous CDEI-binding protein, TFEB, was also tested for CP1 function. The results suggested that DNA binding is required for both biological functions of CP1 but is not sufficient. A direct and quantitative correlation was observed between the chromosome loss and nutritional (i.e., Met) phenotypes of strains carrying loss of function alleles, but qualitatively the chromosome loss phenotype was more sensitive to decreased CP1 expression. The data are consistent with a model in which CP1 performs the same general chromatin-related function at centromeres and MET gene promoters and is normally present in functional excess.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Anal Biochem. 1976 May 7;72:248-54 - PubMed
    1. Proc Natl Acad Sci U S A. 1992 Oct 1;89(19):9010-4 - PubMed
    1. Mol Cell Biol. 1987 Jan;7(1):403-9 - PubMed
    1. J Biol Chem. 1987 Jul 25;262(21):10035-8 - PubMed
    1. Mol Cell Biol. 1988 Jan;8(1):210-25 - PubMed

Publication types

Substances