Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1993 Sep;37(4):240-4.
doi: 10.1007/BF01518517.

In vitro expansion and analysis of T lymphocyte microcultures obtained from the vaccination sites of cancer patients undergoing active specific immunization with autologous Newcastle-disease-virus-modified tumour cells

M Stoeck  1 , C Marland-Noske, M Manasterski, R Zawatzky, S Horn, V Möbus, P Schlag, V Schirrmacher
Affiliations

In vitro expansion and analysis of T lymphocyte microcultures obtained from the vaccination sites of cancer patients undergoing active specific immunization with autologous Newcastle-disease-virus-modified tumour cells

M Stoeck et al. Cancer Immunol Immunother. 1993 Sep.

Abstract

In order to understand further the effects of Newcastle-disease-virus(NDV)-modified tumour vaccines we investigated the feasibility of isolating lymphocytes from the site of injection of patients undergoing postoperative active specific immunization (ASI) with autologous NDV-modified tumour cells. Delayed-type-hypersensitivity(DTH)-like reactions from five cancer patients were surgically removed, minced and the tissue particles were digested with collagenase and DNase. Lymphoid cells recovered were expanded in a highly efficient limiting-dilution analysis system optimized for T cell growth [Moretta et al. (1983) J Exp Med 157: 743] and lymphocyte microcultures (clonal probability > 0.8) could be grown for up to 1 year. Analysis of the microcultures for phenotype and function showed that the majority were positive for CD4 (92%) and TCR alpha beta (96%). Concanavalin-A-induced production of interleukin-2 (IL-2), IL-6, interferon gamma and tumour necrosis factor alpha was detected in more than 70% of the microcultures. Lectin-dependent cytotoxicity was only very rarely observed. The general characteristics of the microcultures obtained support the notion of a DTH-like reaction taking place at the site of tumour cell challenge. The possibility of in vitro expansion and cultivation of T lymphocytes from ASI vaccination sites should help to elucidate further the role of these cells in active specific immunization against autologous tumour cells.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

References

    1. Bier H, Armonat G, Bier J, Schirrmacher V, Ganzer U. Postoperative active-specific immunotherapy of lymph node micrometastasis in a guinea pig tumor model. Otorhinolaryngology. 1989;51:197. - PubMed
    1. Bohle W, Schlag P, Liebrich W, Hohenberger P, Manasterski M, Müller P, Schirrmacher V. Postoperative active specific immunization in colorectal cancer patients with virus-modified autologous tumor-cell vaccine: first clinical results with tumor cell vaccines modified with live but avirulent newcastle disease virus. Cancer. 1990;66:1517. - PubMed
    1. Dvorak HF, Galli SJ, Dvorak AM. Cellular and vascular manifestations of cell-mediated immunity. Hum Pathol. 1986;17:122. - PubMed
    1. Fleischer B. Non-specific propagation of human antigen-dependent T lymphocyte clones. J Immunol Methods. 1988;109:215. - PubMed
    1. Foon KA. Biological response modifiers: the new immunotherapy. Cancer Res. 1989;49:1621. - PubMed

Publication types