doi: 10.1126/science.8342022.
Greater susceptibility to mutations in lagging strand of DNA replication in Escherichia coli than in leading strand
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- PMID: 8342022
- DOI: 10.1126/science.8342022
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Greater susceptibility to mutations in lagging strand of DNA replication in Escherichia coli than in leading strand
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Abstract
Models of DNA replication in Escherichia coli involve an asymmetric DNA polymerase complex that replicates concurrently the leading and the lagging strands of double-stranded DNA. The effect of asymmetry on mutagenesis was tested with pairs of plasmids containing the unidirectional ColE1 origin of replication and a single lesion located in the leading or lagging strand. The lesion used was the covalent adduct that the chemical carcinogen N-2-acetylaminofluorene (AAF) forms with the C-8 position of guanine. Whether SOS was induced or not, mutations arose at about a 20-fold higher frequency when the AAF adduct was located in the lagging strand than when in the leading strand.
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