doi: 10.1126/science.8266103.
Distinct roles for cyclin-dependent kinases in cell cycle control
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- PMID: 8266103
- DOI: 10.1126/science.8266103
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Distinct roles for cyclin-dependent kinases in cell cycle control
Science.
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Abstract
The key cell-cycle regulator Cdc2 belongs to a family of cyclin-dependent kinases in higher eukaryotes. Dominant-negative mutations were used to address the requirement for kinases of this family in progression through the human cell cycle. A dominant-negative Cdc2 mutant arrested cells at the G2 to M phase transition, whereas mutants of the cyclin-dependent kinases Cdk2 and Cdk3 caused a G1 block. The mutant phenotypes were specifically rescued by the corresponding wild-type kinases. These data reveal that Cdk3, in addition to Cdc2 and Cdk2, executes a distinct and essential function in the mammalian cell cycle.
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