ADP-ribosylation factor, a small GTP-dependent regulatory protein, stimulates phospholipase D activity
- PMID: 8261513
- DOI: 10.1016/0092-8674(93)90323-i
ADP-ribosylation factor, a small GTP-dependent regulatory protein, stimulates phospholipase D activity
Abstract
The hydrolysis of phosphatidylcholine by phospholipase D (PLD) results in the production of phosphatidic acid and choline. An assay that uses an exogenous substrate was developed to measure this activity in membranes and solubilized preparations from HL60 cells. A cytosolic factor markedly enhanced PLD activity in membranes and was essential for GTP gamma S-dependent stimulation of an enriched preparation of PLD. The factor was purified to homogeneity from bovine brain cytosol and identified as a member of the ADP-Ribosylation Factor (ARF) subfamily of small G proteins. Subsequently, recombinant myristoylated ARF1 was found to be a better activator of PLD activity than was the nonmyristoylated form. ARF proteins have been implicated recently as factors for regulation of intracellular vesicle traffic. The current finding suggests that PLD activity plays a prominent role in the action of ARF and that ARF may be a key component in the generation of second messengers via phospholipase D.
Comment in
-
ARF signaling: a potential role for phospholipase D in membrane traffic.Cell. 1993 Dec 17;75(6):1045-8. doi: 10.1016/0092-8674(93)90314-g. Cell. 1993. PMID: 8261507 Review. No abstract available.
Similar articles
-
Low molecular weight GTP-binding proteins in HL-60 granulocytes. Assessment of the role of ARF and of a 50-kDa cytosolic protein in phospholipase D activation.J Biol Chem. 1995 Feb 17;270(7):3172-8. doi: 10.1074/jbc.270.7.3172. J Biol Chem. 1995. PMID: 7852400
-
Partial purification and characterization of Arf-sensitive phospholipase D from porcine brain.J Biol Chem. 1995 Jun 23;270(25):14935-43. doi: 10.1074/jbc.270.25.14935. J Biol Chem. 1995. PMID: 7797473
-
Phospholipase D: a downstream effector of ARF in granulocytes.Science. 1994 Jan 28;263(5146):523-6. doi: 10.1126/science.8290961. Science. 1994. PMID: 8290961
-
Regulation of phospholipase D by low molecular weight GTP-binding proteins.J Lipid Mediat Cell Signal. 1996 Sep;14(1-3):223-7. doi: 10.1016/0929-7855(96)00529-9. J Lipid Mediat Cell Signal. 1996. PMID: 8906566 Review.
-
Phospholipase D as an effector for ADP-ribosylation factor in the regulation of vesicular traffic.Chem Phys Lipids. 1999 Apr;98(1-2):141-52. doi: 10.1016/s0009-3084(99)00026-2. Chem Phys Lipids. 1999. PMID: 10358936 Review.
Cited by
-
Regulation of Sperm Capacitation and the Acrosome Reaction by PIP 2 and Actin Modulation.Asian J Androl. 2015 Jul-Aug;17(4):597-600. doi: 10.4103/1008-682X.154305. Asian J Androl. 2015. PMID: 25966627 Free PMC article. Review.
-
Cross-Kingdom Activation of Vibrio Toxins by ADP-Ribosylation Factor Family GTPases.J Bacteriol. 2020 Nov 19;202(24):e00278-20. doi: 10.1128/JB.00278-20. Print 2020 Nov 19. J Bacteriol. 2020. PMID: 32900828 Free PMC article. Review.
-
Endocytosis and vesicular trafficking of immune complexes and activation of phospholipase D by the human high-affinity IgG receptor requires distinct phosphoinositide 3-kinase activities.Biochem J. 1999 Dec 1;344 Pt 2(Pt 2):605-11. Biochem J. 1999. PMID: 10567246 Free PMC article.
-
Functional and physical interactions of the adaptor protein complex AP-4 with ADP-ribosylation factors (ARFs).EMBO J. 2001 Nov 15;20(22):6265-76. doi: 10.1093/emboj/20.22.6265. EMBO J. 2001. PMID: 11707398 Free PMC article.
-
The Arf GTPase-activating protein ASAP1 regulates the actin cytoskeleton.Proc Natl Acad Sci U S A. 2000 Apr 11;97(8):4011-6. doi: 10.1073/pnas.070552297. Proc Natl Acad Sci U S A. 2000. PMID: 10725410 Free PMC article.
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases
