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. 1994 Apr;125(2):239-52.
doi: 10.1083/jcb.125.2.239.

Rab1 and Ca2+ are required for the fusion of carrier vesicles mediating endoplasmic reticulum to Golgi transport

S N Pind  1 , C Nuoffer, J M McCaffery, H Plutner, H W Davidson, M G Farquhar, W E Balch
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Rab1 and Ca2+ are required for the fusion of carrier vesicles mediating endoplasmic reticulum to Golgi transport

S N Pind et al. J Cell Biol. 1994 Apr.

Abstract

Members of the rab/YPT1/SEC4 gene family of small molecular weight GTPases play key roles in the regulation of vesicular traffic between compartments of the exocytic pathway. Using immunoelectron microscopy, we demonstrate that a dominant negative rab1a mutant, rab1a(N124I), defective for guanine nucleotide binding in vitro, leads to the accumulation of vesicular stomatitis virus glycoprotein (VSV-G) in numerous pre-cis-Golgi vesicles and vesicular-tubular clusters containing rab1 and beta-COP, a subunit of the coatomer complex. Similar to previous observations (Balch et al. 1994. Cell. 76:841-852), VSV-G was concentrated nearly 5-10-fold in vesicular carriers that accumulate in the presence of the rab1a(N124I) mutant. VSV-G containing vesicles and vesicular-tubular clusters were also found to accumulate in the presence of a rab1a effector domain peptide mimetic that inhibits endoplasmic reticulum to Golgi transport, as well as in the absence of Ca2+. These results suggest that the combined action of a Ca(2+)-dependent protein and conformational changes associated with the GTPase cycle of rab1 are essential for a late targeting/fusion step controlling the delivery of vesicles to Golgi compartments.

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References

    1. J Biol Chem. 1992 Dec 5;267(34):24363-8 - PubMed
    1. Cell. 1987 Aug 14;50(4):523-34 - PubMed
    1. J Cell Biol. 1993 Jul;122(1):39-51 - PubMed
    1. Nature. 1993 Mar 25;362(6418):318-24 - PubMed
    1. Proc Natl Acad Sci U S A. 1991 Jul 15;88(14):6264-8 - PubMed

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