Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1994 Mar 29;91(7):2723-7.
doi: 10.1073/pnas.91.7.2723.

Cloning and analysis of the Saccharomyces cerevisiae MNN9 and MNN1 genes required for complex glycosylation of secreted proteins

C L Yip  1 , S K Welch, F Klebl, T Gilbert, P Seidel, F J Grant, P J O'Hara, V L MacKay
Affiliations

Cloning and analysis of the Saccharomyces cerevisiae MNN9 and MNN1 genes required for complex glycosylation of secreted proteins

C L Yip et al. Proc Natl Acad Sci U S A. .

Abstract

Proteins secreted by the yeast Saccharomyces cerevisiae are usually modified by the addition at asparagine-linked glycosylation sites of large heterogeneous mannan units that are highly immunogenic. Secreted proteins from mnn1 mnn9 mutant strains, in contrast, have homogeneous Man10GlcNAc2 oligosaccharides that lack the immunogenic alpha 1,3-mannose linkages. We have cloned and sequenced the MNN9 and MNN1 genes, both of which encode proteins with the characteristics of type II membrane proteins. Mnn9p is a membrane-associated protein with unknown function that is required for the addition of the long alpha 1,6-mannose backbone of the complex mannan, whereas Mnn1p is most likely the alpha 1,3-mannosyltransferase located in the Golgi apparatus.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Biochim Biophys Acta. 1966 Nov 15;128(2):400-2 - PubMed
    1. J Biol Chem. 1993 Dec 15;268(35):26338-45 - PubMed
    1. J Biol Chem. 1973 Jul 10;248(13):4660-6 - PubMed
    1. Proc Natl Acad Sci U S A. 1975 Oct;72(10):3912-6 - PubMed
    1. Genetics. 1977 Jan;85(1):23-33 - PubMed

Associated data