Tolerance induction by clonal deletion of CD4+8+ thymocytes in vitro does not require dedicated antigen-presenting cells
- PMID: 8095457
- DOI: 10.1002/eji.1830230315
Tolerance induction by clonal deletion of CD4+8+ thymocytes in vitro does not require dedicated antigen-presenting cells
Abstract
The cellular requirements of T cell tolerance induction in the thymus by clonal deletion was investigated by using an in vitro assay: thymocytes from mice expressing a transgenic TcR specific for lymphocytic choriomeningitis virus (LCMV) and H-2Db were co-cultured with various H-2b cell types as antigen-presenting cells in the presence of the antigenic LCMV peptide. The results revealed that all cell lines examined including embryonic and transformed fibroblasts, melanoma cells, cortical thymic epithelial cells, lymphomas and neuronal cells induced an antigen dose-dependent deletion of CD4+8+ thymocytes. Similarly, highly enriched accessory cell populations from thymus and spleen (macrophages, dendritic and cortical epithelial cells, i.e. thymic nurse cells) could induce antigen-specific depletion of immature CD4+8+ thymocytes. Depending on the cell type examined micromolar to picomolar concentration of LCMV peptide were required to induce deletion. The effectiveness of deletion by the different cell types did not correlate with their major histocompatibility class I expression level; it was, however, influenced by the presence of ICAM-1 adhesion molecules.
Similar articles
-
Deletion of antigen-specific immature thymocytes by dendritic cells requires LFA-1/ICAM interactions.J Immunol. 1992 Mar 15;148(6):1595-603. J Immunol. 1992. PMID: 1347300
-
Clonal deletion of major histocompatibility complex class I-restricted CD4+CD8+ thymocytes in vitro is independent of the CD95 (APO-1/Fas) ligand.Eur J Immunol. 1995 Oct;25(10):2996-9. doi: 10.1002/eji.1830251043. Eur J Immunol. 1995. PMID: 7589104
-
Clonal deletion of immature CD4+8+ thymocytes in suspension culture by extrathymic antigen-presenting cells.Nature. 1991 May 9;351(6322):150-3. doi: 10.1038/351150a0. Nature. 1991. PMID: 1903182
-
T cell tolerance and antigen presenting cell function in the thymus.Adv Exp Med Biol. 1993;329:159-64. doi: 10.1007/978-1-4615-2930-9_27. Adv Exp Med Biol. 1993. PMID: 8379366 Review.
-
Antigen processing and presentation in the thymus: implications for T cell repertoire selection.Curr Opin Immunol. 2017 Jun;46:53-57. doi: 10.1016/j.coi.2017.03.014. Epub 2017 May 3. Curr Opin Immunol. 2017. PMID: 28477557 Review.
Cited by
-
CD4 T cell tolerance to human C-reactive protein, an inducible serum protein, is mediated by medullary thymic epithelium.J Exp Med. 1998 Jul 6;188(1):5-16. doi: 10.1084/jem.188.1.5. J Exp Med. 1998. PMID: 9653079 Free PMC article.
-
Quantitative impact of thymic clonal deletion on the T cell repertoire.J Exp Med. 1997 Feb 3;185(3):377-83. doi: 10.1084/jem.185.3.377. J Exp Med. 1997. PMID: 9053438 Free PMC article.
-
Galectin-1, an endogenous lectin produced by thymic epithelial cells, induces apoptosis of human thymocytes.J Exp Med. 1997 May 19;185(10):1851-8. doi: 10.1084/jem.185.10.1851. J Exp Med. 1997. PMID: 9151710 Free PMC article.
-
An acetylcholine receptor alpha subunit promoter confers intrathymic expression in transgenic mice. Implications for tolerance of a transgenic self-antigen and for autoreactivity in myasthenia gravis.J Clin Invest. 1998 Jun 1;101(11):2340-50. doi: 10.1172/JCI1615. J Clin Invest. 1998. PMID: 9616205 Free PMC article.
-
Positive selection of self- and alloreactive CD8+ T cells in Tap-1 mutant mice.Proc Natl Acad Sci U S A. 1994 Jul 5;91(14):6525-8. doi: 10.1073/pnas.91.14.6525. Proc Natl Acad Sci U S A. 1994. PMID: 8022816 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Research Materials
Miscellaneous
