Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1994 Aug 1;13(15):3638-47.
doi: 10.1002/j.1460-2075.1994.tb06671.x.

Three ARS elements contribute to the ura4 replication origin region in the fission yeast, Schizosaccharomyces pombe

D D Dubey  1 , J Zhu, D L Carlson, K Sharma, J A Huberman
Affiliations

Three ARS elements contribute to the ura4 replication origin region in the fission yeast, Schizosaccharomyces pombe

D D Dubey et al. EMBO J. .

Abstract

The ura4 replication origin region, which is located near the ura4 gene on chromosome III of the fission yeast, Schizosaccharomyces pombe, contains multiple initiation sites. We have used 2D gel electrophoretic replicon mapping methods to study the distribution of these initiation sites, and have found that they are concentrated near three ARS elements (stretches of DNA which permit autonomous plasmid replication). To determine the roles of these ARS elements in the function of the ura4 origin region, we deleted either one or two of them from the chromosome and then assessed the consequences of the deletions by 2D gel electrophoresis. The results suggest that each of the three ARS elements is responsible for the initiation events in its vicinity and that the ARS elements interfere with each other in a hierarchical fashion. It is possible that the large initiation zones of animal cells are similarly composed of multiple mutually interfering origins.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Mol Gen Genet. 1988 Dec;215(1):81-6 - PubMed
    1. Mol Cell Biol. 1992 Oct;12(10):4733-41 - PubMed
    1. Genetics. 1989 May;122(1):19-27 - PubMed
    1. Cell. 1989 Jun 16;57(6):909-20 - PubMed
    1. Nucleic Acids Res. 1990 Feb 11;18(3):647-52 - PubMed

Publication types

MeSH terms