Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1994 Sep;68(9):5439-47.
doi: 10.1128/JVI.68.9.5439-5447.1994.

Intramolecular recombination in polyomavirus DNA is a nonconservative process directed from the viral intergenic region

C Nault  1 , A Fricker, L Delbecchi, D Bourgaux-Ramoisy, P Bourgaux
Affiliations

Intramolecular recombination in polyomavirus DNA is a nonconservative process directed from the viral intergenic region

C Nault et al. J Virol. 1994 Sep.

Abstract

Previously, we have studied intramolecular homologous recombination in polyomavirus replicons under conditions allowing only one amplifiable recombination product to be generated from a single precursor molecule. In order to detect putative reciprocal product(s), we have now constructed precursor polyomavirus replicons which contain two copies, instead of one copy, of the viral intergenic region, including the origin of replication as well as both promoters. Upon transfection of mouse cells, constructs containing directly repeated intergenic regions yielded distinct amplifiable products, in number depending upon the functional integrity of both intergenic regions. Our data indicate that of two possible reciprocal products, a given precursor molecule would yield either one or the other but never both at the same time. Most striking, however, is the observation that promoter function is required for recombination, while the origin of replication function may be needed only for amplification of the recombination product once it has been formed. The data reported here confirm and extend previous data suggesting that (i) transcription is instrumental in recombination between direct repeats and (ii) nonconservative recombination involving direct repeats relies upon two promoters of opposing polarities.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

References

    1. Cold Spring Harb Symp Quant Biol. 1975;39 Pt 1:45-52 - PubMed
    1. J Mol Biol. 1967 Jun 14;26(2):365-9 - PubMed
    1. Proc Natl Acad Sci U S A. 1979 Sep;76(9):4571-5 - PubMed
    1. Experientia. 1981 Oct 15;37(10):1074-5 - PubMed
    1. Virology. 1982 Oct 15;122(1):84-97 - PubMed

Publication types

LinkOut - more resources