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. 1994 Jul;97(1):39-44.
doi: 10.1111/j.1365-2249.1994.tb06576.x.

Co-expression of the CD45RA and CD45RO antigens on T lymphocytes in chronic arthritis

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Co-expression of the CD45RA and CD45RO antigens on T lymphocytes in chronic arthritis

K L Summers et al. Clin Exp Immunol. 1994 Jul.

Abstract

The site of T lymphocyte activation in chronic arthritis is unknown. Peripheral blood (PB) lymphocytes from chronic arthritis patients are in a 'naive' or non-activated state, as defined by expression of the CD45RA antigen and lack of HLA class II expression. In contrast, most synovial fluid (SF) T lymphocytes express a 'memory' or activated phenotype, as defined by the CD45RO antigen and high HLA class II expression. Following stimulation, naive cells lose CD45RA and gain CD45RO expression to become memory cells with a transitional stage of dual CD45RA, CD45RO antigen expression. To localize where this change in phenotype occurs we used dual colour immunofluorescence labelling to compare the percentage of dual CD45RA, CD45RO-positive T lymphocytes in PB and SF from chronic arthritic patients and from normal PB, assuming this population would be increased at the primary site of T lymphocyte activation. Expression of the intermediate and late activation marker, HLA-DR, was also analysed using dual colour immunofluorescence labelling. The percentage of dual positive T lymphocytes was similar between arthritic PB, SF, and normal PB, as was the density of both CD45RA and CD45RO antigens. Thus, CD45 isoform expression did not indicate where T lymphocytes were activated. However, we identified a previously unreported population of CD45RA+ CD45RO+ HLA-DR- T lymphocytes in arthritic and normal PB. In SF, this population was absent, but a substantial number of dual CD45RA, CD45RO-positive HLA-DR+ T lymphocytes were identified. This population would not be predicted by the current model of T lymphocyte activation. Division of T lymphocytes into functional groups on the basis of CD45 isoform expression is likely to be more complicated than previously thought. Based on our findings we propose an alternative model of T lymphocyte differentiation.

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References

    1. J Exp Med. 1979 Aug 1;150(2):246-55 - PubMed
    1. Pathology. 1993 Apr;25(2):167-73 - PubMed
    1. J Immunol. 1985 Jun;134(6):3762-9 - PubMed
    1. Immunology. 1986 May;58(1):63-70 - PubMed
    1. Immunology. 1987 Jul;61(3):351-6 - PubMed

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