Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1994 Dec;14(12):7704-16.
doi: 10.1128/mcb.14.12.7704-7716.1994.

The basic helix-loop-helix-zipper domain of TFE3 mediates enhancer-promoter interaction

S E Artandi  1 , C Cooper, A Shrivastava, K Calame
Affiliations

The basic helix-loop-helix-zipper domain of TFE3 mediates enhancer-promoter interaction

S E Artandi et al. Mol Cell Biol. 1994 Dec.

Abstract

Binding sites for three families of sequence-specific DNA-binding proteins, microE3, C/EBP, and OCT, are found in both the promoters and the intronic enhancer of the immunoglobulin heavy-chain gene. We have used a cotransfection system to investigate how proteins binding these sites may participate in enhancer-promoter interactions. Basic helix-loop-helix-zipper (BHLHZIP) proteins TFE3 and TFEB activate from a distance in this assay, but the basic zipper (BZIP) protein NF-IL6 and endogenous OCT-binding proteins do not. Our results suggest that remotely bound TFE3 is recruited to the initiation site by association with proximally bound TFE3; this interaction is mediated by the BHLHZIP domain and not by activation domains of TFE3. The BZIP domain of Ig/EBP lacks this activity, revealing an important functional difference between these structurally related dimerization domains. We also show that TFE3 can exist as a tetramer in solution and that tetramerization is determined by the HLHZIP domain. These data support a model in which protein-protein interactions between proximally and remotely bound TFE3 recruit TFE3 to the initiation site for activation. The IgH gene is the first example of a cellular gene in which proximal and distal binding sites are found for a protein capable of mediating enhancer-promoter interaction.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. J Mol Biol. 1990 Jul 20;214(2):373-80 - PubMed
    1. Genes Dev. 1990 Aug;4(8):1404-15 - PubMed
    1. Prog Nucleic Acid Res Mol Biol. 1990;39:81-128 - PubMed
    1. Genes Dev. 1990 Oct;4(10):1730-40 - PubMed
    1. Science. 1990 Nov 23;250(4984):1149-51 - PubMed

Publication types

MeSH terms

Substances

LinkOut - more resources