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. 1994 Oct 7;266(5182):117-9.
doi: 10.1126/science.7939629.

Identification of the ron gene product as the receptor for the human macrophage stimulating protein

M H Wang  1 , C Ronsin, M C Gesnel, L Coupey, A Skeel, E J Leonard, R Breathnach
Affiliations

Identification of the ron gene product as the receptor for the human macrophage stimulating protein

M H Wang et al. Science. .

Abstract

Macrophage-stimulating protein (MSP) is a member of the hepatocyte growth factor-scatter factor (HGF-SF) family. Labeled MSP bound to Madin-Darby canine kidney (MDCK) cells transfected with complementary DNA encoding Ron, a cell membrane protein tyrosine kinase. Cross-linking of 125I-labeled MSP to transfected cells (MDCK-RE7 cells) and immunoprecipitation by antibodies to Ron revealed a 220-kilodalton complex, a size consistent with that of MSP (80 kilodaltons) cross-linked to the beta chain of Ron (150 kilodaltons). The binding of 125I-labeled MSP to MDCK-RE7 cells was inhibited by unlabeled MSP, but not by HGF-SF. MSP caused phosphorylation of the beta chain of Ron and induced migration of MDCK-RE7 cells. These results establish the ron gene product as a specific cell-surface receptor for MSP.

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