Induction of long-lasting immunity to Plasmodium yoelii malaria with whole blood-stage antigens and copolymer adjuvants
- PMID: 7836760
Induction of long-lasting immunity to Plasmodium yoelii malaria with whole blood-stage antigens and copolymer adjuvants
Abstract
We previously reported that protection of mice from nonlethal Plasmodium yoelii malaria by immunization with whole killed blood-stage parasites was dependent on the adjuvant and that adjuvants influenced both the specificity and isotype of Ab. Additional studies with the most effective formulations were undertaken to better define the protective responses and 100% protection from lethal P. yoelii malaria was produced by three immunizations with Ag in copolymer P1004 and detoxified RaLPS as adjuvants and 83% protection was induced by a single immunization. The protection lasted for 9 mo and was associated with an anamnestic rise in Ab titer of the IgG2a isotype during the challenge infection. Passive immunization with Ab from animals that had been immunized and challenged transferred sterile immunity. Splenectomy reduced, but did not abolish, protection. These data suggest that the effective Ab is directed against labile epitopes on the surface of blood-stage parasites. The vaccines primed animals for production of such Ab, but its synthesis was efficiently induced only by challenge with live organisms.
Similar articles
-
Role of adjuvants in the modulation of antibody isotype, specificity, and induction of protection by whole blood-stage Plasmodium yoelii vaccines.J Immunol. 1993 Dec 15;151(12):7077-85. J Immunol. 1993. PMID: 8258712
-
Induction of protective polyclonal antibodies by immunization with a Plasmodium yoelii circumsporozoite protein multiple antigen peptide vaccine.J Immunol. 1995 Mar 15;154(6):2784-93. J Immunol. 1995. PMID: 7876549
-
Multiple antigen constructs (MACs): induction of sterile immunity against sporozoite stage of rodent malaria parasites, Plasmodium berghei and Plasmodium yoelii.Vaccine. 1997 Apr;15(5):482-8. doi: 10.1016/s0264-410x(96)00301-5. Vaccine. 1997. PMID: 9160515
-
Copolymer adjuvants in malaria vaccine development.Am J Trop Med Hyg. 1994;50(4 Suppl):52-8. doi: 10.4269/ajtmh.1994.50.52. Am J Trop Med Hyg. 1994. PMID: 8172332 Review.
-
Liver or blood-stage arrest during malaria sporozoite immunization: the later the better?Trends Parasitol. 2013 Jun;29(6):304-10. doi: 10.1016/j.pt.2013.03.008. Epub 2013 Apr 20. Trends Parasitol. 2013. PMID: 23608185 Review.
Cited by
-
Immunomodulator effect of picroliv and its potential in treatment against resistant Plasmodium yoelii (MDR) infection in mice.Pharm Res. 2008 Oct;25(10):2312-9. doi: 10.1007/s11095-008-9631-2. Epub 2008 Jun 13. Pharm Res. 2008. PMID: 18551251
-
Cytokines and antibody subclass associated with protective immunity against blood-stage malaria in mice vaccinated with the C terminus of merozoite surface protein 1 plus a novel adjuvant.Infect Immun. 1996 Sep;64(9):3532-6. doi: 10.1128/iai.64.9.3532-3536.1996. Infect Immun. 1996. PMID: 8751895 Free PMC article.
-
Partial protection against Plasmodium vivax blood-stage infection in Saimiri monkeys by immunization with a recombinant C-terminal fragment of merozoite surface protein 1 in block copolymer adjuvant.Infect Immun. 1999 Jan;67(1):342-9. doi: 10.1128/IAI.67.1.342-349.1999. Infect Immun. 1999. PMID: 9864235 Free PMC article.
-
Why functional pre-erythrocytic and bloodstage malaria vaccines fail: a meta-analysis of fully protective immunizations and novel immunological model.PLoS One. 2010 May 19;5(5):e10685. doi: 10.1371/journal.pone.0010685. PLoS One. 2010. PMID: 20502667 Free PMC article.
-
Protective immunity against Plasmodium yoelii malaria induced by immunization with particulate blood-stage antigens.Infect Immun. 1997 Aug;65(8):3138-45. doi: 10.1128/iai.65.8.3138-3145.1997. Infect Immun. 1997. PMID: 9234766 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Medical