Learning about DNA polymerase function by studying antimutator DNA polymerases
- PMID: 7770910
- DOI: 10.1016/s0968-0004(00)88987-2
Learning about DNA polymerase function by studying antimutator DNA polymerases
Abstract
Mutant bacteriophage T4 DNA polymerases exist that appear primarily to reduce the frequency of AT-to-GC transitions when this [antimutator' phenotype is assessed by genetic methods. This observation disagrees with in vitro studies, which indicate that T4 antimutator DNA polymerases have increased proofreading abilities and effectively edit all types of base substitution errors. One explanation that reconciles the apparent in vivo mutational specificity of antimutator DNA polymerases with their biochemical properties is that the in vivo mutational specificity identifies mismatched primer-termini that are corrected less efficiently by the wild-type level of proofreading activity, but are corrected if proofreading is increased.
Similar articles
-
Motif A of bacteriophage T4 DNA polymerase: role in primer extension and DNA replication fidelity. Isolation of new antimutator and mutator DNA polymerases.J Biol Chem. 1994 Feb 25;269(8):5635-43. J Biol Chem. 1994. PMID: 8119900
-
Regulation of DNA polymerase exonucleolytic proofreading activity: studies of bacteriophage T4 "antimutator" DNA polymerases.Genetics. 1998 Apr;148(4):1551-7. doi: 10.1093/genetics/148.4.1551. Genetics. 1998. PMID: 9560374 Free PMC article. Review. No abstract available.
-
Genetic and biochemical studies of bacteriophage T4 DNA polymerase 3'-->5'-exonuclease activity.J Biol Chem. 1993 Dec 25;268(36):27100-8. J Biol Chem. 1993. PMID: 8262948
-
Selection of bacteriophage T4 antimutator DNA polymerases: a link between proofreading and sensitivity to phosphonoacetic acid.Mutat Res. 1996 Feb 19;350(1):9-16. doi: 10.1016/0027-5107(95)00085-2. Mutat Res. 1996. PMID: 8657202
-
DNA polymerase fidelity: from genetics toward a biochemical understanding.Genetics. 1998 Apr;148(4):1475-82. doi: 10.1093/genetics/148.4.1475. Genetics. 1998. PMID: 9560367 Free PMC article. Review.
Cited by
-
John W. (Jan) Drake: A Biochemical View of a Geneticist Par Excellence.Genetics. 2020 Dec;216(4):827-836. doi: 10.1534/genetics.120.303813. Genetics. 2020. PMID: 33268388 Free PMC article.
-
Substitution of a residue contacting the triphosphate moiety of the incoming nucleotide increases the fidelity of yeast DNA polymerase zeta.Nucleic Acids Res. 2008 Mar;36(5):1731-40. doi: 10.1093/nar/gkn023. Epub 2008 Feb 7. Nucleic Acids Res. 2008. PMID: 18263611 Free PMC article.
-
Identification of a new motif in family B DNA polymerases by mutational analyses of the bacteriophage t4 DNA polymerase.J Mol Biol. 2010 Jul 16;400(3):295-308. doi: 10.1016/j.jmb.2010.05.030. Epub 2010 May 21. J Mol Biol. 2010. PMID: 20493878 Free PMC article.
-
DNA replication fidelity in Escherichia coli: a multi-DNA polymerase affair.FEMS Microbiol Rev. 2012 Nov;36(6):1105-21. doi: 10.1111/j.1574-6976.2012.00338.x. Epub 2012 Apr 5. FEMS Microbiol Rev. 2012. PMID: 22404288 Free PMC article. Review.
-
Enhanced polymerase activity permits efficient synthesis by cancer-associated DNA polymerase ϵ variants at low dNTP levels.Nucleic Acids Res. 2022 Aug 12;50(14):8023-8040. doi: 10.1093/nar/gkac602. Nucleic Acids Res. 2022. PMID: 35822874 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Miscellaneous
