Antisense oligoribonucleotides and RNase P. A great potential
- PMID: 7684934
- DOI: 10.1016/0300-9084(93)90035-q
Antisense oligoribonucleotides and RNase P. A great potential
Abstract
This paper presents the possible--but at present mostly hypothetical--applications of RNase P for the specific inactivation of target RNAs. The natural substrates for RNase P are pre-tRNAs. The enzyme can also recognize and cleave smaller model substrates. These are simple hairpins for bacterial RNase P whereas the requirements for eukaryotic RNase P are more complex and less well understood. It is possible to split the RNase P substrates into two separate RNA molecules. One part of the split substrate RNA contains the RNase P cleavage site and the 5'-terminal half of the acceptor stem, embedded in a large target RNA. The other part of the substrate RNA provides the 3'-terminal half of the acceptor stem and it serves as antisense sequence ('external guide sequence'). Both parts can hybridize and reconstitute a functional substrate for RNase P; this results in cleavage of the target RNA by RNase P. The properties of this system are presented and advantages and problems discussed.
Similar articles
-
Short oligonucleotides as external guide sequences for site-specific cleavage of RNA molecules with human RNase P.RNA. 1998 Jul;4(7):847-55. doi: 10.1017/s1355838298980323. RNA. 1998. PMID: 9671057 Free PMC article.
-
Design of short external guide sequences (EGSs) for cleavage of target molecules with RNase P.Nucleic Acids Symp Ser. 1997;(36):19-21. Nucleic Acids Symp Ser. 1997. PMID: 9478194
-
Rational design of self-cleaving pre-tRNA-ribonuclease P RNA conjugates.Biochemistry. 1994 Sep 6;33(35):10800-8. doi: 10.1021/bi00201a030. Biochemistry. 1994. PMID: 8075082
-
RNase P RNA-mediated catalysis.Biochem Soc Trans. 2002 Nov;30(Pt 6):1153-8. doi: 10.1042/bst0301153. Biochem Soc Trans. 2002. PMID: 12440994 Review.
-
Ribonuclease P: unity and diversity in a tRNA processing ribozyme.Annu Rev Biochem. 1998;67:153-80. doi: 10.1146/annurev.biochem.67.1.153. Annu Rev Biochem. 1998. PMID: 9759486 Review.
Cited by
-
Short oligonucleotides as external guide sequences for site-specific cleavage of RNA molecules with human RNase P.RNA. 1998 Jul;4(7):847-55. doi: 10.1017/s1355838298980323. RNA. 1998. PMID: 9671057 Free PMC article.
-
Further characterization of human RNase MRP/RNase P and related autoantibodies.Mol Biol Rep. 1998 Mar;25(2):95-101. doi: 10.1023/a:1006878511162. Mol Biol Rep. 1998. PMID: 9540070
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
