Kinetic study on the production and degradation of leupeptin in Streptomyces exfoliatus SMF13
- PMID: 7662340
- DOI: 10.1016/0168-1656(95)00061-t
Kinetic study on the production and degradation of leupeptin in Streptomyces exfoliatus SMF13
Abstract
Medium composition and cultivation conditions were constructed for the optimum production of leupeptin by Streptomyces exfoliatus SMF13. The production of leupeptin was related to mycelial growth, being optimum in the cultivation with glucose-excess, phosphate-limited, and casamino acids medium. However, leupeptin-inactivating enzyme (LIE) was produced in the cultivation with glucose-limited, phosphate-excess, and Na-caseinate medium where mycelium degradation was accompanied. LIE was one of the most important factors in optimizing the leupeptin productivity. Kinetic parameters calculated from batch and chemostat cultivations revealed that qlpt was closely related to qs and mu, but qLIE was increased after mu declined to near zero, and followed by kd. Optimum production process of leupeptin was determined with phosphate-limited continuous cultivation, which did not permit LIE production. The maximum productivity (0.24 g l-1 h-1) and production yield (1.64 g leupeptin per g glucose) of phosphate-limited chemostat cultivation were 2.4- and 4-times larger than those of batch cultivation, respectively. This is the first cultivation kinetic analysis for leupeptin production and its inactivation by LIE in relation to mycelium differentiation.
Similar articles
-
Trypsin-like protease of Streptomyces exfoliatus SMF13, a potential agent in mycelial differentiation.Microbiology (Reading). 1996 Jul;142 ( Pt 7):1797-806. doi: 10.1099/13500872-142-7-1797. Microbiology (Reading). 1996. PMID: 8757742
-
Characterization of the leupeptin-inactivating enzyme from Streptomyces exfoliatus SMF13 which produces leupeptin.Biochem J. 1998 Apr 15;331 ( Pt 2)(Pt 2):539-45. doi: 10.1042/bj3310539. Biochem J. 1998. PMID: 9531495 Free PMC article.
-
Physiological roles of leupeptin and extracellular proteases in mycelium development of Streptomyces exfoliatus SMF13.Microbiology (Reading). 1995 Apr;141 ( Pt 4):1017-25. doi: 10.1099/13500872-141-4-1017. Microbiology (Reading). 1995. PMID: 7773379
-
Preparation and purification of the proteinase inhibitor, leupeptin, from culture filtrates of Streptomyces lavendulae.Int J Biochem. 1986;18(9):813-20. doi: 10.1016/0020-711x(86)90058-3. Int J Biochem. 1986. PMID: 3758463
-
Biosynthesis of leupeptin. IV. Is protein turnover in leupeptin producer cells affected by leupeptin?J Antibiot (Tokyo). 1980 Oct;33(10):1172-6. doi: 10.7164/antibiotics.33.1172. J Antibiot (Tokyo). 1980. PMID: 7451369
Cited by
-
Mechanism of Microbial Metabolite Leupeptin in the Treatment of COVID-19 by Traditional Chinese Medicine Herbs.mBio. 2021 Oct 26;12(5):e0222021. doi: 10.1128/mBio.02220-21. Epub 2021 Sep 28. mBio. 2021. PMID: 34579576 Free PMC article.
-
Screening of wild type Streptomyces isolates able to overproduce clavulanic acid.Braz J Microbiol. 2014 Oct 9;45(3):919-28. doi: 10.1590/s1517-83822014000300022. eCollection 2014. Braz J Microbiol. 2014. PMID: 25477926 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Research Materials
