Heparin-induced release of extracellular-superoxide dismutase form (V) to plasma
- PMID: 7629026
- DOI: 10.1093/oxfordjournals.jbchem.a124748
Heparin-induced release of extracellular-superoxide dismutase form (V) to plasma
Abstract
Extracellular-superoxide dismutase [EC 1.15.1.1] (EC-SOD) is a secretory, tetrameric glycoprotein. A prominent feature of EC-SOD is its affinity for heparin. This enzyme in serum is heterogeneous with regard to heparin-affinity and can be divided into five fractions (I) to (V) by heparin-HPLC, whereas fibroblast-secreted EC-SOD consists mainly of form (V). An intravenous injection of 50 i.u. of heparin/kg body weight into two healthy volunteers led to an immediate rise of serum EC-SOD level by 2.4-2.8-fold. Only form (V), which was a minor component in pre-heparin serum, was increased by the intravenous injection. The half-life of serum EC-SOD after the prompt rise was about 90 min. The in vivo experiment using rats and an in vitro experiment strongly suggested the EC-SOD released into the plasma reconstituted the interaction with glycocalyx on the vascular endothelial cell surface in accordance with the elimination of heparin from the vascular system.
Similar articles
-
Changes in the heparin affinity of extracellular-superoxide dismutase in patients with coronary artery atherosclerosis.Biol Pharm Bull. 1998 Oct;21(10):1090-3. doi: 10.1248/bpb.21.1090. Biol Pharm Bull. 1998. PMID: 9821816
-
Heparin-induced release of extracellular superoxide dismutase to human blood plasma.Biochem J. 1987 Feb 15;242(1):55-9. doi: 10.1042/bj2420055. Biochem J. 1987. PMID: 3593249 Free PMC article.
-
Extracellular superoxide dismutase in the vascular system of mammals.Biochem J. 1988 Oct 1;255(1):223-8. Biochem J. 1988. PMID: 3196315 Free PMC article.
-
[Extracellular superoxide dismutase (EC-SOD)--structure, properties and functions].Postepy Hig Med Dosw (Online). 2004 Jul 24;58:301-11. Postepy Hig Med Dosw (Online). 2004. PMID: 15280800 Review. Polish.
-
Extracellular superoxide dismutase.Int J Biochem Cell Biol. 2005 Dec;37(12):2466-71. doi: 10.1016/j.biocel.2005.06.012. Epub 2005 Jul 21. Int J Biochem Cell Biol. 2005. PMID: 16087389 Review.
Cited by
-
Prevention of adverse events of interferon γ gene therapy by gene delivery of interferon γ-heparin-binding domain fusion protein in mice.Mol Ther Methods Clin Dev. 2014 Jun 18;1:14023. doi: 10.1038/mtm.2014.23. eCollection 2014. Mol Ther Methods Clin Dev. 2014. PMID: 26015966 Free PMC article.
-
Extracellular superoxide dismutase (ecSOD) in vascular biology: an update on exogenous gene transfer and endogenous regulators of ecSOD.Transl Res. 2008 Feb;151(2):68-78. doi: 10.1016/j.trsl.2007.10.003. Epub 2007 Nov 8. Transl Res. 2008. PMID: 18201674 Free PMC article. Review.
-
Heparin and Heparin-Derivatives in Post-Subarachnoid Hemorrhage Brain Injury: A Multimodal Therapy for a Multimodal Disease.Molecules. 2017 May 2;22(5):724. doi: 10.3390/molecules22050724. Molecules. 2017. PMID: 28468328 Free PMC article. Review.
-
Compartmental Cerebrospinal Fluid Events Occurring after Subarachnoid Hemorrhage: An "Heparin Oriented" Systematic Review.Int J Mol Sci. 2023 Apr 25;24(9):7832. doi: 10.3390/ijms24097832. Int J Mol Sci. 2023. PMID: 37175544 Free PMC article. Review.
-
Carcinogenesis and Reactive Oxygen Species Signaling: Interaction of the NADPH Oxidase NOX1-5 and Superoxide Dismutase 1-3 Signal Transduction Pathways.Antioxid Redox Signal. 2019 Jan 20;30(3):443-486. doi: 10.1089/ars.2017.7268. Epub 2018 Nov 22. Antioxid Redox Signal. 2019. PMID: 29478325 Free PMC article. Review.
