doi: 10.1111/j.1365-2672.1995.tb05031.x.
The ability of membrane potential dyes and calcafluor white to distinguish between viable and non-viable bacteria
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- PMID: 7537262
- DOI: 10.1111/j.1365-2672.1995.tb05031.x
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The ability of membrane potential dyes and calcafluor white to distinguish between viable and non-viable bacteria
J Appl Bacteriol.
1995 Mar.
Abstract
Various dyes were assessed for their ability to discriminate between viable and non-viable bacteria. Two methods of killing were employed: by heat treatment or by gramicidin treatment. Staining was carried out in two ways; by staining directly in the medium or by washing cells prior to staining in buffer. Carbocyanine and rhodamine 123 dyes only exhibited small changes in fluorescence between viable and non-viable populations of bacteria. Both oxonol dye (bis 1,3-dibutylbarbituric acid trimethine oxonol) and calcafluor white proved much more useful.
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