Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1995 Nov 15;312 ( Pt 1)(Pt 1):63-7.
doi: 10.1042/bj3120063.

Changes in the components of a nuclear inositide cycle during differentiation in murine erythroleukaemia cells

N Divecha  1 , A J Letcher, H H Banfic, S G Rhee, R F Irvine
Affiliations

Changes in the components of a nuclear inositide cycle during differentiation in murine erythroleukaemia cells

N Divecha et al. Biochem J. .

Abstract

Differentiation of murine erythroleukaemia cells with the chemical agent DMSO leads to a cessation of proliferation and the production of a number of erythrocyte markers such as haemoglobin. We have previously demonstrated that activation of proliferation leads to an increase in the production of nuclear diacylglycerol (DAG). Here we demonstrate that differentiation leads to a decrease in the levels of nuclear DAG and the activity of the nuclear-associated phosphoinositidase C (PIC). The change in activity appears to be due to a decrease in the mass levels of the beta 1 isoform, as demonstrated by the use of isoform-specific antibodies. Moreover, the changes correlate with the cessation of proliferation and an increase in the number of cells in G1 phase of the cell cycle, rather than with the number of cells which have differentiated. Indeed, although treatment of the cells with phorbol 12-myristate 13-acetate (PMA) inhibits the differentiation programme as assessed by haemoglobin staining, it does not inhibit the number of cells blocking in G1 of the cell cycle or the changes in nuclear DAG or PIC activity. The possible involvement of this nuclear inositide cycle during progression through the cell cycle is discussed.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. J Cell Biol. 1968 Mar;36(3):551-65 - PubMed
    1. Cancer Res. 1994 May 15;54(10):2536-40 - PubMed
    1. Proc Natl Acad Sci U S A. 1971 Feb;68(2):378-82 - PubMed
    1. Nature. 1976 Jul 29;262(5567):353-6 - PubMed
    1. Biochim Biophys Acta. 1980 Sep 22;605(3):325-46 - PubMed

Publication types

MeSH terms

Substances