Members of a family of proteins (the RD family) detected by a U1 70K monoclonal antibody are present in spliceosomal complexes
- PMID: 7479068
- PMCID: PMC307346
- DOI: 10.1093/nar/23.20.4081
Members of a family of proteins (the RD family) detected by a U1 70K monoclonal antibody are present in spliceosomal complexes
Abstract
We have characterized a monoclonal antibody (mAb) to the U1 snRNP component U1 70K. We find that this antibody recognizes several proteins, in addition to U1 70K, in purified spliceosomal complexes and in total HeLa cell nuclear extract preparations. The novel mAb U1 70K antigens can also be specifically immunoprecipitated by the antibody. Similarly to U1 70K, many of the mAb U1 70K antigens can be phosphorylated by a co-purifying kinase activity. The epitope recognized by mAb U1 70K was previously shown to be a repeating arginine/aspartate (RD) dipeptide. Thus we have designated the novel mAb U1 70K antigens the RD family. Comparison of mAb U1 70K with a recently characterized antibody, mAb 16H3, whose epitope is a repeating R/D or R/E motif, showed that a large subset of the antigens are common. In contrast, most of the mAb U1 70K antigens are distinct from the proteins detected by mAb 104, an antibody to the SR family of splicing factors.
Similar articles
-
A conserved epitope on a subset of SR proteins defines a larger family of Pre-mRNA splicing factors.J Cell Biol. 1995 May;129(4):899-908. doi: 10.1083/jcb.129.4.899. J Cell Biol. 1995. PMID: 7538140 Free PMC article.
-
CLK1 reorganizes the splicing factor U1-70K for early spliceosomal protein assembly.Proc Natl Acad Sci U S A. 2021 Apr 6;118(14):e2018251118. doi: 10.1073/pnas.2018251118. Proc Natl Acad Sci U S A. 2021. PMID: 33811140 Free PMC article.
-
Identification of a novel, non-snRNP protein complex containing U1A protein.RNA. 1997 Dec;3(12):1444-55. RNA. 1997. PMID: 9404895 Free PMC article.
-
The plant U1 small nuclear ribonucleoprotein particle 70K protein interacts with two novel serine/arginine-rich proteins.Plant Cell. 1998 Oct;10(10):1637-48. doi: 10.1105/tpc.10.10.1637. Plant Cell. 1998. PMID: 9761791 Free PMC article.
-
Arabidopsis U1 snRNP 70K protein and its interacting proteins: nuclear localization and in vivo dynamics of a novel plant-specific serine/arginine-rich protein.Symp Soc Exp Biol. 2004;(56):279-95. Symp Soc Exp Biol. 2004. PMID: 15565888 Review. No abstract available.
Cited by
-
Luc7p, a novel yeast U1 snRNP protein with a role in 5' splice site recognition.Genes Dev. 1999 Sep 15;13(18):2425-38. doi: 10.1101/gad.13.18.2425. Genes Dev. 1999. PMID: 10500099 Free PMC article.
-
Association of polyadenylation cleavage factor I with U1 snRNP.RNA. 2003 Nov;9(11):1400-9. doi: 10.1261/rna.5104603. RNA. 2003. PMID: 14561889 Free PMC article.
-
Association of phosphorylated serine/arginine (SR) splicing factors with the U1-small ribonucleoprotein (snRNP) autoantigen complex accompanies apoptotic cell death.J Exp Med. 1998 Feb 16;187(4):547-60. doi: 10.1084/jem.187.4.547. J Exp Med. 1998. PMID: 9463405 Free PMC article.
-
The Patterning and Proportion of Charged Residues in the Arginine-Rich Mixed-Charge Domain Determine the Membrane-Less Organelle Targeted by the Protein.Int J Mol Sci. 2022 Jul 11;23(14):7658. doi: 10.3390/ijms23147658. Int J Mol Sci. 2022. PMID: 35887012 Free PMC article.
-
Evidence that U5 snRNP recognizes the 3' splice site for catalytic step II in mammals.EMBO J. 1997 Aug 1;16(15):4746-59. doi: 10.1093/emboj/16.15.4746. EMBO J. 1997. PMID: 9303319 Free PMC article.
References
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Research Materials
Miscellaneous
