A screening method for isolating DNA repair-deficient mutants of CHO cells
- PMID: 7404270
- DOI: 10.1007/BF01542791
A screening method for isolating DNA repair-deficient mutants of CHO cells
Abstract
A simple procedure for isolating mutagen-sensitive clones of CHO cells was developed and applied in mutant hunts in which colonies were screened for hypersensitivity to killing by ultraviolet radiation (UV, ethyl methanesulfonate (EMS), or mitomycin C (MMC). Each of two UV-sensitive clones studied in detail had a D37 dose of 1.0 J/m2 compared to 7.0 J/m2 for the wild-type cells, and each was shown to have no detectable repair replication following exposure to UV doses of up to 26 J/m2. Although these mutants resemble xeroderma pigmentosum human mutants with respect to their repair defect and cross-sensitivity to the carcinogen 4-nitroquinoline-1-oxide, one of two clones (UV-20) is characterized by extreme hypersensitivity to MMC (80-fold as compared to the wild type). Clones having hypersensitivity to alkylating agents, but not UV, were obtained using MMC and EMS. In the latter case the two clones had significantly increased sensitivity to the killing action of 60Co gamma-rays.
Similar articles
-
Isolation and cross-sensitivity of X-ray-sensitive mutants of V79-4 hamster cells.Mutat Res. 1987 May;183(3):279-86. doi: 10.1016/0167-8817(87)90011-3. Mutat Res. 1987. PMID: 3106801
-
Large-scale isolation of UV-sensitive clones of CHO cells.Somatic Cell Genet. 1980 May;6(3):407-18. doi: 10.1007/BF01542792. Somatic Cell Genet. 1980. PMID: 7404271
-
Sensitivity to DNA-damaging agents and mutation induction by UV light in UV-sensitive CHO cells.Mutat Res. 1986 Jun;174(2):155-9. doi: 10.1016/0165-7992(86)90108-9. Mutat Res. 1986. PMID: 3086715
-
Cytogenetical characterization of UV-sensitive repair-deficient CHO cell line 43-3B. II. Induction of cell killing, chromosomal aberrations and sister-chromatid exchanges by 4NQO, mono- and bi-functional alkylating agents.Mutat Res. 1989 Jun;212(2):103-12. doi: 10.1016/0027-5107(89)90061-4. Mutat Res. 1989. PMID: 2499773
-
[Postreplicative DNA repair in UV-sensitive Chinese hamster cell clones].Tsitologiia. 1980 Sep;22(9):1085-95. Tsitologiia. 1980. PMID: 7003862 Review. Russian.
Cited by
-
Role of Human N-Acetyltransferase 2 Genetic Polymorphism on Aromatic Amine Carcinogen-Induced DNA Damage and Mutagenicity in a Chinese Hamster Ovary Cell Mutation Assay.Environ Mol Mutagen. 2020 Feb;61(2):235-245. doi: 10.1002/em.22331. Epub 2019 Sep 30. Environ Mol Mutagen. 2020. PMID: 31490564 Free PMC article.
-
Mutations in hamster single-strand break repair gene XRCC1 causing defective DNA repair.Nucleic Acids Res. 1998 Feb 15;26(4):1032-7. doi: 10.1093/nar/26.4.1032. Nucleic Acids Res. 1998. PMID: 9461464 Free PMC article.
-
Intracellular generation of ROS by 3,5-dimethylaminophenol: persistence, cellular response, and impact of molecular toxicity.Toxicol Sci. 2014 Sep;141(1):300-13. doi: 10.1093/toxsci/kfu127. Epub 2014 Jun 27. Toxicol Sci. 2014. PMID: 24973092 Free PMC article.
-
Azacytidine-induced reactivation of a DNA repair gene in Chinese hamster ovary cells.Mol Cell Biol. 1986 Aug;6(8):2944-9. doi: 10.1128/mcb.6.8.2944-2949.1986. Mol Cell Biol. 1986. PMID: 2431295 Free PMC article.
-
Postreplication repair and the susceptibility of Chinese hamster cells to cytotoxic and mutagenic effects of alkylating agents.Proc Natl Acad Sci U S A. 1980 Oct;77(10):6072-6. doi: 10.1073/pnas.77.10.6072. Proc Natl Acad Sci U S A. 1980. PMID: 6934532 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Research Materials