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. 1980 Jun;34(3):598-603.
doi: 10.1128/JVI.34.3.598-603.1980.

Defective mutant of Sindbis virus with a smaller-molecular-weight form of the E1 glycoprotein

A Leone, V Colantuoni, G Pontarelli, R Cancedda

Defective mutant of Sindbis virus with a smaller-molecular-weight form of the E1 glycoprotein

A Leone et al. J Virol. 1980 Jun.

Abstract

We have isolated from a single plaque a mutant of Sindbis virus characterized by an E1 glycoprotein with higher electrophoretic mobility. This higher mobility is not attributable to a different extent of glycosylation of the protein nor to an altered proteolytic maturation pathway of the polypeptide precursor, but is the result of a deletion occurring during the replication of the viral RNA. The 26S RNA (the messenger for the Sindbis structural proteins) extracted from cells infected with the mutant is about 0.75 x 10(5) daltons smaller than the 26S RNA from the parental strain. As a consequence, in cells infected with the mutant, an E1 glycoprotein is synthesized with a polypeptide chain about 70 amino acids shorter. The biological relevance of this naturally occurring deletion of the viral genome is discussed.

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References

    1. Virology. 1966 Oct;30(2):204-13 - PubMed
    1. Nature. 1970 Aug 15;227(5259):680-5 - PubMed
    1. J Antibiot (Tokyo). 1971 Nov;24(11):785-94 - PubMed
    1. Virology. 1972 May;48(2):615-7 - PubMed
    1. J Virol. 1972 Nov;10(5):925-32 - PubMed