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. 1980 Mar;47(1):215-20.
doi: 10.1099/0022-1317-47-1-215.

A protein, VPg, covalently linked to 36S calicivirus RNA

A protein, VPg, covalently linked to 36S calicivirus RNA

F L Schaffer et al. J Gen Virol. 1980 Mar.

Abstract

Proteins associated with 36S virus RNA from Vero cells infected with San Miguel sea lion virus, type 2 (SMSV-2), were labelled with 125I. One protein, VPg, remained linked to RNA when subjected to deproteinization techniques. VPg labelled wtih 32P was observed on 36S RNA from purified virions; the quantity of label was compatible with two phosphates per genome. The estimated mol. wt. of SMSV-2 VPg was 15 000.

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