Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1981;83(4):493-505.
doi: 10.1007/BF00328275.

Anaphase progression and furrow establishment in nocodazole-arrested PtK1 cells

J M Mullins, J A Snyder

Anaphase progression and furrow establishment in nocodazole-arrested PtK1 cells

J M Mullins et al. Chromosoma. 1981.

Abstract

The relationship between progression through anaphase and furrow establishment was investigated in PtK1 cells using the anti-mitotic agent Nocodazole to arrest cells at different points in anaphase. The capacity of cells to furrow was compared to the kinetochore-kinetochore separation attained at the time of arrest. For the stages of anaphase examined, furrowing capacity increased directly with kinetochore-kinetochore separation until complete furrows were formed after kinetochore-kinetochore separations of 14 micron or more were reached. Furrow establishment thus occurs during a definite interval during anaphase in PtK1 cells. Results from electron microscopy of both Nocodazole-treated and control cells suggest that a population of astral microtubules may be important for furrow establishment.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

References

    1. Exp Cell Res. 1980 Apr;126(2):397-405 - PubMed
    1. J Cell Biol. 1976 May;69(2):443-54 - PubMed
    1. Biochem Biophys Res Commun. 1976 Mar 22;69(2):319-24 - PubMed
    1. J Exp Zool. 1961 Oct;148:81-9 - PubMed
    1. Exp Cell Res. 1973 Apr;79(1):95-105 - PubMed

Publication types

LinkOut - more resources