[Changes in the lipid makeup of isolated metaphase chromosomes with their proteins and DNA removed]
[Article in
Russian]
- PMID: 7256846
Item in Clipboard
[Changes in the lipid makeup of isolated metaphase chromosomes with their proteins and DNA removed]
[Article in
Russian]
Tsitologiia.
1981 Apr.
Abstract
Metaphase chromosomes of HeLa cells were isolated by a modified method of Wray and Stubblefield. The isolated chromosomes were treated with 0.2 N HCl-Triton X-100 and DNAse I. Most of the polar lipids are removed from the chromosomes together with acid soluble proteins, whereas neutral lipids, except cholesterol esters, are removed with DNA. The mode of interaction between chromosome lipids, DNA and proteins is discussed.
Similar articles
-
[Relationship between the lipid composition of metaphase chromosomes and the method of isolating them].Tsitologiia. 1981 Feb;23(2):185-91. Tsitologiia. 1981. PMID: 7256841 Russian. No abstract available.
-
Dissociation of lipid and protein from cell-DNA by direct alkaline sucrose gradient analysis.Jpn J Exp Med. 1975 Jun;45(3):215-22. Jpn J Exp Med. 1975. PMID: 1177361
-
[Chromosome fractionation. II. Features of the composition and behavior of biphasic polymer systems of metaphase chromosomes obtained from HeLa cells at acidic and neutral pH values].Tsitologiia. 1981 Feb;23(2):192-204. Tsitologiia. 1981. PMID: 6167036 Russian.
-
Chromosome mediated gene transfer.Annu Rev Biochem. 1981;50:533-54. doi: 10.1146/annurev.bi.50.070181.002533. Annu Rev Biochem. 1981. PMID: 7023365 Review. No abstract available.
-
The chromosome periphery during mitosis.Bioessays. 1994 Mar;16(3):179-85. doi: 10.1002/bies.950160308. Bioessays. 1994. PMID: 8166671 Review.