In vivo and in vitro translation of the RNAs of four tobamoviruses
- PMID: 7251334
- DOI: 10.1159/000149199
In vivo and in vitro translation of the RNAs of four tobamoviruses
Abstract
The RNAs from four tobamoviruses [tobacco mosaic virus(TMV) vulgare, TMV dahlemense, TMV U2, and the cowpea strain of TMV (CcTMV)] were translated in a cell-free ribosome system from reticulocytes. Among the translation products found were two polypeptides with molecular weights of 170,000 and 120,000 which were produced by all the viral RNAs tested. These two polypeptides comigrated with the corresponding virus-specific proteins synthesized in tobacco protoplasts infected with any of the four tobamoviruses. A 30,000 molecular weight protein was encoded by all four RNAs. However, this protein was synthesized in different amounts and differed slightly in size for each virus. When TMV vulgare or dahlemense RNAs were used as templates, this protein was one of the major translation products, whereas trace amounts were produced in the presence of U2 or CcTMV RNA. Only the CcTMV RNA directed the in vitro synthesis of a protein which had about the same electrophoretic mobility as coat protein.
Similar articles
-
The expression of the TMV-specific 30-kDa protein in tobacco protoplasts is strongly and selectively enhanced by actinomycin.Virology. 1989 Mar;169(1):51-61. doi: 10.1016/0042-6822(89)90040-8. Virology. 1989. PMID: 2466372
-
In vitro translation of polyribosome-associated RNAs from tobamovirus-infected plants.Intervirology. 1976;7(4-5):256-71. doi: 10.1159/000149958. Intervirology. 1976. PMID: 1010713
-
Nucleotide sequence and translation of satellite tobacco mosaic virus RNA.Virology. 1989 May;170(1):139-46. doi: 10.1016/0042-6822(89)90361-9. Virology. 1989. PMID: 2718378
-
Elucidation of the genome organization of tobacco mosaic virus.Philos Trans R Soc Lond B Biol Sci. 1999 Mar 29;354(1383):587-91. doi: 10.1098/rstb.1999.0410. Philos Trans R Soc Lond B Biol Sci. 1999. PMID: 10212938 Free PMC article. Review.
-
Replication of tobacco mosaic virus RNA.Philos Trans R Soc Lond B Biol Sci. 1999 Mar 29;354(1383):613-27. doi: 10.1098/rstb.1999.0413. Philos Trans R Soc Lond B Biol Sci. 1999. PMID: 10212941 Free PMC article. Review.
Cited by
-
Stop making sense: or Regulation at the level of termination in eukaryotic protein synthesis.FEBS Lett. 1988 Aug 1;235(1-2):1-15. doi: 10.1016/0014-5793(88)81225-0. FEBS Lett. 1988. PMID: 3042454 Free PMC article. Review.
-
The leaky UGA termination codon of tobacco rattle virus RNA is suppressed by tobacco chloroplast and cytoplasmic tRNAs(Trp) with CmCA anticodon.EMBO J. 1992 Nov;11(11):4167-73. doi: 10.1002/j.1460-2075.1992.tb05510.x. EMBO J. 1992. PMID: 1396598 Free PMC article.
-
UAG readthrough during TMV RNA translation: isolation and sequence of two tRNAs with suppressor activity from tobacco plants.EMBO J. 1984 Feb;3(2):351-6. doi: 10.1002/j.1460-2075.1984.tb01810.x. EMBO J. 1984. PMID: 16453503 Free PMC article.
-
Analysis of in vitro and in vivo products of the TMV 30kDa open reading frame using antisera raised against a synthetic peptide.FEBS Lett. 1983 Dec 12;164(2):355-360. doi: 10.1016/0014-5793(83)80316-0. FEBS Lett. 1983. PMID: 33785967 Free PMC article.
-
Pseudouridine in the anticodon G psi A of plant cytoplasmic tRNA(Tyr) is required for UAG and UAA suppression in the TMV-specific context.Nucleic Acids Res. 1992 Nov 25;20(22):5911-8. doi: 10.1093/nar/20.22.5911. Nucleic Acids Res. 1992. PMID: 1461724 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
