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. 1978 Oct;5(10):3843-54.
doi: 10.1093/nar/5.10.3843.

Semi-conservative transcription in particles of a double-stranded RNA mycovirus

G Ratti, K W Buck
Free PMC article

Semi-conservative transcription in particles of a double-stranded RNA mycovirus

G Ratti et al. Nucleic Acids Res. 1978 Oct.
Free PMC article

Abstract

During transcription in vitro catalysed by the virion RNA polymerase of Aspergillus foetidus virus AfV-S in the presence of tritiated UTP, the virus double-stranded RNA becomes labelled in one strand, which has the same sequence as the single-stranded RNA transcripts produced. Most of the label incorporated into double-stranded RNA could be chased into single stranded RNA by further reaction with excess unlabelled nucleoside triphosphates. In reactions containing tritiated UTP the single-stranded RNA transcripts released after the first round of transcription were unlabelled. It is concluded that transcription in virions of AfV-S occurs by displacement of one of the strands of double-stranded RNA by the RNA strand being newly synthesised i.e. the reaction is semi-conservative with respect to double-stranded RNA.

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