doi: 10.1093/nar/5.10.3665.
Reactions at the termini of tRNA with T4 RNA ligase
- PMID: 724498
- PMCID: PMC342702
- DOI: 10.1093/nar/5.10.3665
Item in Clipboard
Reactions at the termini of tRNA with T4 RNA ligase
Nucleic Acids Res.
1978 Oct.
Free PMC article
Abstract
T4 RNA ligase will catalyze the addition of nucleoside 3', 5'-bisphosphates onto the 3' terminus of tRNA resulting in tRNA molecule one nucleotide longer with a 3' terminal phosphate. Under appropriate conditions the reaction is quantitative and, if high specific radioactivity bisphosphates are used, it provides an efficient means for in vitro labeling of tRNA. Although the 3' terminal hydroxyl is a good acceptor, the 5' terminal phosphate in most tRNA's is not an effective donor in the RNA ligase reaction. This poor reactivity is due to the secondary structure of the 5' terminal nucleotide. If E. Coli tRNAf Met is used, the 5' phosphate is reactive and the major product with RNA ligase is the cyclic tRNA.
Similar articles
-
T4 RNA Ligase joins 2'-deoxyribonucleoside 3',5'-bisphosphates to oligodeoxyribonucleotides.Biochemistry. 1978 Nov 28;17(24):5091-7. doi: 10.1021/bi00617a004. Biochemistry. 1978. PMID: 728390
-
Enzymatic oligoribonucleotide synthesis with T4 RNA ligase.Biochemistry. 1978 May 30;17(11):2069-76. doi: 10.1021/bi00604a008. Biochemistry. 1978. PMID: 667012
-
Addition of mononucleotides to oligoribonucleotide acceptors with T4 RNA ligase.Proc Natl Acad Sci U S A. 1978 Mar;75(3):1270-3. doi: 10.1073/pnas.75.3.1270. Proc Natl Acad Sci U S A. 1978. PMID: 274717 Free PMC article.
-
T4 RNA ligase as a nucleic acid synthesis and modification reagent.Gene Amplif Anal. 1981;2:313-45. Gene Amplif Anal. 1981. PMID: 6765649 Review. No abstract available.
-
Diversity and roles of (t)RNA ligases.Cell Mol Life Sci. 2012 Aug;69(16):2657-70. doi: 10.1007/s00018-012-0944-2. Epub 2012 Mar 17. Cell Mol Life Sci. 2012. PMID: 22426497 Free PMC article. Review.
Cited by
-
Effect of the higher-order structure of tRNAs on the stability of hybrids with oligodeoxyribonucleotides: separation of tRNA by an efficient solution hybridization.Nucleic Acids Res. 1992 May 11;20(9):2223-32. doi: 10.1093/nar/20.9.2223. Nucleic Acids Res. 1992. PMID: 1594442 Free PMC article.
-
Direct chemical method for sequencing RNA.Proc Natl Acad Sci U S A. 1979 Apr;76(4):1760-4. doi: 10.1073/pnas.76.4.1760. Proc Natl Acad Sci U S A. 1979. PMID: 377283 Free PMC article.
-
Characterization of elongating T7 and SP6 RNA polymerases and their response to a roadblock generated by a site-specific DNA binding protein.Nucleic Acids Res. 1991 Sep 11;19(17):4639-46. doi: 10.1093/nar/19.17.4639. Nucleic Acids Res. 1991. PMID: 1891355 Free PMC article.
-
3' Terminal labelling of RNA of RNA with beta-32P-pyrophosphate group and its application to the sequence analysis of 5S RNA from Streptomyces griseus.Nucleic Acids Res. 1980 Sep 25;8(18):4111-24. doi: 10.1093/nar/8.18.4111. Nucleic Acids Res. 1980. PMID: 6159585 Free PMC article.
-
HIV-1 reverse transcriptase-associated RNase H cleaves RNA/RNA in arrested complexes: implications for the mechanism by which RNase H discriminates between RNA/RNA and RNA/DNA.EMBO J. 1995 Feb 15;14(4):833-41. doi: 10.1002/j.1460-2075.1995.tb07061.x. EMBO J. 1995. PMID: 7533725 Free PMC article.
References
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Miscellaneous
