Cellular and molecular heterogeneity of H-2Kk antigens
- PMID: 7229421
- DOI: 10.1111/j.1744-313x.1981.tb00747.x
Cellular and molecular heterogeneity of H-2Kk antigens
Abstract
Up to 70% of mouse spleen cells expressing H-2Kk alloantigens reacted with the monoclonal anti-H-2Kk antibody 11-4.1 in an indirect rosetting assay. When the cells that rosetted with the monoclonal antibody 11-4.1 were removed by density centrifugation, the residual unreactive cells reacted with the polyclonal anti-H-2Kk alloantiserum D23 but not with the monoclonal antibody 11-4.1 indicating the existence of two cel populations. In sequential immunoprecipitation experiments, the glycoprotein fraction of NP40 extracts from H-2Kk cells, after removal of antigens reacting with the monoclonal antibody 11-4.1 still contained structures reactive with the alloantiserum. Therefore, there are at least two antigenically distinct H-2Kk molecules which are differentially expressed on two subpopulations of spleen cells.
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