Specific interaction of one subunit of eukaryotic initiation factor eIF-3 with 18S ribosomal RNA within the binary complex, eIF-3 small ribosomal subunit, as shown by cross-linking experiments
- PMID: 7071015
- PMCID: PMC320528
- DOI: 10.1093/nar/10.4.1327
Specific interaction of one subunit of eukaryotic initiation factor eIF-3 with 18S ribosomal RNA within the binary complex, eIF-3 small ribosomal subunit, as shown by cross-linking experiments
Abstract
Initiation factor eIF-3 from rat liver forms a binary complex with the small ribosomal subunit. Within this complex, 18S ribosomal RNA can be cross-linked to the 66 000 dalton subunit of eIF-3 by treating the complex with a short bifunctional reagent, diepoxybutane, with a distance of 4A between the reactive groups. In binary complexes containing eIF-3 premodified with the heterobifunctional reagent, methyl-p-azido-benzoylaminoacetimidate (10A), the 66 000 dalton subunit of eIF-3 became covalently bound to 18S rRNA after irradiation of the complex with ultraviolet light. The involvement of only one of the eight eIF-3 subunits in the formation of the covalent RNA-protein complexes indicates a highly specific interaction between 18S rRNA and eIF-3 at the attachment site of the factor on the 40S subunit.
Similar articles
-
Cross-linking of Met-tRNAf to eIF-2 beta and to the ribosomal proteins S3a and S6 within the eukaryotic inhibition complex, eIF-2 .GMPPCP.Met-tRNAf.small ribosomal subunit.Nucleic Acids Res. 1981 May 25;9(10):2387-96. doi: 10.1093/nar/9.10.2387. Nucleic Acids Res. 1981. PMID: 6910637 Free PMC article.
-
The alpha and gamma subunits of initiation factor eIF-2 can be cross-linked to 18S ribosomal RNA within the quaternary initiation complex, eIF-2.Met-tRNAf.GDPCP.small ribosomal subunit.Nucleic Acids Res. 1980 Jul 25;8(14):3065-71. doi: 10.1093/nar/8.14.3065. Nucleic Acids Res. 1980. PMID: 7443514 Free PMC article.
-
The spatial arrangement of the complex between eukaryotic initiation factor eIF-3 and 40 S ribosomal subunit. Cross-linking between factor and ribosomal proteins.Biochim Biophys Acta. 1983 Oct 13;741(1):103-8. doi: 10.1016/0167-4781(83)90015-5. Biochim Biophys Acta. 1983. PMID: 6615839
-
Eukaryotic initiation factors eIF-2 and eIF-3: interactions, structure and localization in ribosomal initiation complexes.Biochimie. 1991 Jul-Aug;73(7-8):1007-19. doi: 10.1016/0300-9084(91)90142-n. Biochimie. 1991. PMID: 1742346 Review.
-
Participation of initiation factors in the recruitment of mRNA to ribosomes.Biochimie. 1994;76(9):831-8. doi: 10.1016/0300-9084(94)90184-8. Biochimie. 1994. PMID: 7880899 Review.
Cited by
-
Computational modeling of eukaryotic mRNA turnover.RNA. 2001 Sep;7(9):1192-212. doi: 10.1017/s1355838201010330. RNA. 2001. PMID: 11565744 Free PMC article.
-
mTOR-dependent stimulation of the association of eIF4G and eIF3 by insulin.EMBO J. 2006 Apr 19;25(8):1659-68. doi: 10.1038/sj.emboj.7601047. Epub 2006 Mar 16. EMBO J. 2006. PMID: 16541103 Free PMC article.
-
Ribosomal position and contacts of mRNA in eukaryotic translation initiation complexes.EMBO J. 2008 Jun 4;27(11):1609-21. doi: 10.1038/emboj.2008.90. Epub 2008 May 8. EMBO J. 2008. PMID: 18464793 Free PMC article.
-
Binding of eukaryotic initiation factor 3 to ribosomal 40S subunits and its role in ribosomal dissociation and anti-association.RNA. 2005 Apr;11(4):470-86. doi: 10.1261/rna.7215305. Epub 2005 Feb 9. RNA. 2005. PMID: 15703437 Free PMC article.
-
Identification by RNA-protein cross-linking of ribosomal proteins located at the interface between the small and the large subunits of mammalian ribosomes.EMBO J. 1982;1(3):357-62. doi: 10.1002/j.1460-2075.1982.tb01174.x. EMBO J. 1982. PMID: 6201358 Free PMC article.
References
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
