Inhibition of myoblast differentiation in vitro by a protein isolated from liver cell medium
- PMID: 7047537
- PMCID: PMC2112867
- DOI: 10.1083/jcb.93.2.395
Inhibition of myoblast differentiation in vitro by a protein isolated from liver cell medium
Abstract
We have recently discovered that cells of Coon's Buffalo rat liver (BRL) line secrete a protein which is a potent inhibitor of skeletal myoblast differentiation in vitro. Using ion exchange and molecular exclusion chromatography, we have prepared this protein, which we designate "differentiation inhibitor" (DI), from the materials secreted by BRL cells maintained in serum-free medium. It is a relatively heat-stable protein which is inactivated by treatment with trypsin and mercaptoethanol and has an apparent molecular weight in the range 30,000--36,000. It exhibits no detectable mitogenic or lectin activity and differs from previously reported inhibitors of myoblast differentiation in several respects. It is active in all skeletal myoblast systems tested (Yaffe's L6 line as well as primary cultures of rat, chick, and Japanese quail myoblasts), and it blocks fusion, elevation of creatine kinase, and increased binding of alpha-bungarotoxin. Parallel fractionation of fetal bovine serum (FBS) and chick embryo extract (CEE) yields a peak of activity which similarly inhibits myoblast differentiation. We suggest that the differentiation inhibitor from BRL cells may correspond to the differentiation-inhibiting component(s) of FBS and CEE, and we call attention to the possibility that such a substance could play a role in embryonic growth of myoblasts and in satellite cell formation.
Similar articles
-
Stimulation and inhibition of myoblast differentiation by hormones.In Vitro. 1984 Dec;20(12):942-58. doi: 10.1007/BF02619668. In Vitro. 1984. PMID: 6397421
-
Transforming growth factor-beta. A very potent inhibitor of myoblast differentiation, identical to the differentiation inhibitor secreted by Buffalo rat liver cells.J Biol Chem. 1986 Dec 15;261(35):16509-13. J Biol Chem. 1986. PMID: 3465726
-
Hormonal stimulation of myoblast differentiation in the absence of DNA synthesis.Am J Physiol. 1982 Nov;243(5):C278-84. doi: 10.1152/ajpcell.1982.243.5.C278. Am J Physiol. 1982. PMID: 6753603
-
Biphasic concentration dependency of stimulation of myoblast differentiation by somatomedins.Am J Physiol. 1986 May;250(5 Pt 1):C771-8. doi: 10.1152/ajpcell.1986.250.5.C771. Am J Physiol. 1986. PMID: 3518483
-
Growth hormone and the insulin-like growth factor system in myogenesis.Endocr Rev. 1996 Oct;17(5):481-517. doi: 10.1210/edrv-17-5-481. Endocr Rev. 1996. PMID: 8897022 Review.
Cited by
-
Adipocyte conversion of CHEF cells in serum-free medium.J Cell Biol. 1985 Feb;100(2):429-34. doi: 10.1083/jcb.100.2.429. J Cell Biol. 1985. PMID: 3881457 Free PMC article.
-
Type beta transforming growth factor is an inhibitor of myogenic differentiation.Proc Natl Acad Sci U S A. 1986 Nov;83(21):8206-10. doi: 10.1073/pnas.83.21.8206. Proc Natl Acad Sci U S A. 1986. PMID: 3022285 Free PMC article.
-
Stimulation and inhibition of myoblast differentiation by hormones.In Vitro. 1984 Dec;20(12):942-58. doi: 10.1007/BF02619668. In Vitro. 1984. PMID: 6397421
-
Regulation of myogenic differentiation by type beta transforming growth factor.J Cell Biol. 1986 Nov;103(5):1799-805. doi: 10.1083/jcb.103.5.1799. J Cell Biol. 1986. PMID: 3465734 Free PMC article.
-
Control by fibroblast growth factor of differentiation in the BC3H1 muscle cell line.J Cell Biol. 1985 May;100(5):1540-7. doi: 10.1083/jcb.100.5.1540. J Cell Biol. 1985. PMID: 3988800 Free PMC article.
References
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources
Other Literature Sources
Miscellaneous