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. 1981 Dec;34(3):987-92.
doi: 10.1128/iai.34.3.987-992.1981.

Detection of an immediate early herpes simplex virus type 1 polypeptide in trigeminal ganglia from latently infected animals

M T Green, R J Courtney, E C Dunkel

Detection of an immediate early herpes simplex virus type 1 polypeptide in trigeminal ganglia from latently infected animals

M T Green et al. Infect Immun. 1981 Dec.

Abstract

In this study, trigeminal sensory ganglia from animals with acute herpes simplex virus, type 1 (HSV-1) infection were compared to those with a latent infection for the expression of HSV-specific antigens. By the indirect immunofluorescence assay, antisera to an immediate early polypeptide of molecular weight 175,000, designated VP175 or ICP4, and a hyperimmune antiserum to HSV-1 were used to determine whether early viral polypeptides were being expressed in neurons during the latent stage of infection. All 17 ganglia from animals with acute infection (sacrificed 3 to 12 days postinfection) exhibited positive staining when treated either with anti-HSV-1 or with anti-VP175. Forty of 42 ganglia from animals sacrificed during the latent stage of infection (22 to 200 days postinfection) exhibited immunofluorescent staining when treated with anti-VP175. The staining appeared to be similar to that observed in ganglia from acutely infected animals stained with anti-VP175, except that the number and distribution of stained cells were markedly reduced. No immunofluorescence was observed in ganglia from noninfected control animals when stained with anti-VP175 or anti-HSV-1, or when ganglia from latently infected animals were stained with anti-HSV-1 or preimmune serum.

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References

    1. JAMA. 1969 Jun 2;208(9):1675-9 - PubMed
    1. Science. 1971 Aug 27;173(3999):843-5 - PubMed
    1. Nat New Biol. 1972 Feb 16;235(59):216-7 - PubMed
    1. N Engl J Med. 1973 Mar 29;288(13):648-50 - PubMed
    1. Infect Immun. 1973 Feb;7(2):272-88 - PubMed

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