The gcr (glycolysis regulation) mutation of Saccharomyces cerevisiae
- PMID: 7031056
The gcr (glycolysis regulation) mutation of Saccharomyces cerevisiae
Abstract
gcr is a mutation considerably decreasing the assayed amounts of most glycolysis enzymes in Saccharomyces cerevisiae (Clifton, D., Weinstock, S. B., and Fraenkel, D. G. (1978) Genetics 88, 1-11). We show here that although in the wild type strain the amounts of these enzymes do not greatly differ between cells from different media, in the gcr mutant strain most of the enzyme amounts are 5% or less, relative to wild type, from cells grown without sugars, but 20-50% from cells grown with sugars. Lower relative values were found for phosphoglycerate mutase and enolase. A corresponding alteration in the mutant in the intensities of several major protein bands could even be seen in stained gels after electrophoresis of crude extracts: the profiles were otherwise normal. Results of titration of phosphoglycerate kinase with antibody accorded with activity. Transfer of cells between the two types of media did not lead to a more rapid adjustment of enzyme amounts than expected from the steady state levels. gcr is not allelic to GPM (the gene for phosphoglycerate mutase) or to RNA1 (which affects transport of RNA from the nucleus). Translation of total RNA in a rabbit reticulocyte lysate gave a pattern of polypeptides similar to the in vivo one. Thus, gcr is likely to affect somehow mRNA synthesis or lifetime for a discrete number of proteins.
Similar articles
-
Human acylphosphatase cannot replace phosphoglycerate kinase in Saccharomyces cerevisiae.Antonie Van Leeuwenhoek. 2001 Oct;80(1):11-7. doi: 10.1023/a:1012082312137. Antonie Van Leeuwenhoek. 2001. PMID: 11761363
-
Perturbation of phosphoglycerate kinase 1 (PGK1) only marginally affects glycolysis in cancer cells.J Biol Chem. 2020 May 8;295(19):6425-6446. doi: 10.1074/jbc.RA119.012312. Epub 2020 Mar 26. J Biol Chem. 2020. PMID: 32217690 Free PMC article.
-
Transcription of the phosphoglycerate kinase gene of Saccharomyces cerevisiae increases when fermentative cultures are stressed by heat-shock.Eur J Biochem. 1986 Dec 15;161(3):525-31. doi: 10.1111/j.1432-1033.1986.tb10474.x. Eur J Biochem. 1986. PMID: 3539592
-
Influence of low glycolytic activities in gcr1 and gcr2 mutants on the expression of other metabolic pathway genes in Saccharomyces cerevisiae.Yeast. 2005 Jan 30;22(2):111-27. doi: 10.1002/yea.1198. Yeast. 2005. PMID: 15645478
-
Simultaneous overexpression of enzymes of the lower part of glycolysis can enhance the fermentative capacity of Saccharomyces cerevisiae.Yeast. 2000 Oct;16(14):1325-34. doi: 10.1002/1097-0061(200010)16:14<1325::AID-YEA627>3.0.CO;2-E. Yeast. 2000. PMID: 11015729
Cited by
-
The DNA-binding protein RAP1 is required for efficient transcriptional activation of the yeast PYK glycolytic gene.Curr Genet. 1990 Dec;18(5):405-12. doi: 10.1007/BF00309909. Curr Genet. 1990. PMID: 2127735
-
A multi-component upstream activation sequence of the Saccharomyces cerevisiae glyceraldehyde-3-phosphate dehydrogenase gene promoter.Mol Gen Genet. 1991 Dec;231(1):22-32. doi: 10.1007/BF00293817. Mol Gen Genet. 1991. PMID: 1753943
-
A PAS Protein Directs Metabolic Reprogramming during Cryptococcal Adaptation to Hypoxia.mBio. 2021 Mar 16;12(2):e03602-20. doi: 10.1128/mBio.03602-20. mBio. 2021. PMID: 33727360 Free PMC article.
-
Identification of an upstream activation site in the pyruvate decarboxylase structural gene (PDC1) of Saccharomyces cerevisiae.Curr Genet. 1988 Nov;14(5):405-12. doi: 10.1007/BF00521261. Curr Genet. 1988. PMID: 3147147
-
Characterization of TPI gene expression in isogeneic wild-type and gcr1-deletion mutant strains of Saccharomyces cerevisiae.Nucleic Acids Res. 1990 Dec 11;18(23):7099-107. doi: 10.1093/nar/18.23.7099. Nucleic Acids Res. 1990. PMID: 2263469 Free PMC article.
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources
Molecular Biology Databases
