Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1981 Jun;78(6):3433-7.
doi: 10.1073/pnas.78.6.3433.

recA protein of Escherichia coli promotes branch migration, a kinetically distinct phase of DNA strand exchange

M M Cox, I R Lehman

recA protein of Escherichia coli promotes branch migration, a kinetically distinct phase of DNA strand exchange

M M Cox et al. Proc Natl Acad Sci U S A. 1981 Jun.

Abstract

The recA protein of Escherichia coli promotes the complete exchange of strands between full-length linear duplex and single-stranded circular DNA molecules of bacteriophage phi X-174, converting more than 50% of the single-stranded DNA into heteroduplex replicative form II-like structures. Kinetically, the reaction can be divided into two phases, formation of short heteroduplex regions (D loops) and extension of the D loops via branch migration. recA protein participates directly in both phases. D loops are formed efficiently in the presence of ATP or the nonhydrolyzable ATP analog adenosine 5'-[gamma-thio]triphosphate, whereas D-loop extension requires continuous ATP hydrolysis. Complete strand exchange requires a stoichiometric amount of recA protein and is strongly stimulated by the single-stranded-DNA-binding protein of E. coli.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. J Mol Biol. 1970 Feb 28;48(1):1-22 - PubMed
    1. Proc Natl Acad Sci U S A. 1981 Apr;78(4):2100-4 - PubMed
    1. Annu Rev Genet. 1973;7:67-86 - PubMed
    1. Annu Rev Microbiol. 1974;28(0):445-68 - PubMed
    1. Proc Natl Acad Sci U S A. 1976 Jul;73(7):2299-303 - PubMed

Publication types

LinkOut - more resources