doi: 10.1073/pnas.79.15.4560.
Flipping of cloned d(pCpG)n.d(pCpG)n DNA sequences from right- to left-handed helical structure by salt, Co(III), or negative supercoiling
- PMID: 6956879
- PMCID: PMC346714
- DOI: 10.1073/pnas.79.15.4560
Item in Clipboard
Flipping of cloned d(pCpG)n.d(pCpG)n DNA sequences from right- to left-handed helical structure by salt, Co(III), or negative supercoiling
Proc Natl Acad Sci U S A.
1982 Aug.
Abstract
Negative supercoiling of plasmid DNAs containing 24--42 base pairs of alternating d(pCpG) inserts is shown to cause the flipping of the helical hand of the inserts from right to left under physiological conditions. For a negatively supercoiled DNA with a fixed linking number, this flipping reduces its superhelicity and, therefore, is accompanied by a shift of its electrophoretic mobility in agarose gel. Quantitation of the mobility shifts indicates that essentially the whole stretch of contiguous alternating d(pCpG) flips to the left-handed helical form when the negative superhelical density (specific linking difference) is greatest than or equal to 0.03.
Similar articles
-
Rate of B to Z structural transition of supercoiled DNA.J Mol Biol. 1986 Jul 5;190(1):125-7. doi: 10.1016/0022-2836(86)90082-3. J Mol Biol. 1986. PMID: 3783696
-
Energetics of B-to-Z transition in DNA.Proc Natl Acad Sci U S A. 1983 Oct;80(20):6206-10. doi: 10.1073/pnas.80.20.6206. Proc Natl Acad Sci U S A. 1983. PMID: 6578505 Free PMC article.
-
Conformational flexibility of junctions between contiguous B- and Z-DNAs in supercoiled plasmids.Proc Natl Acad Sci U S A. 1983 May;80(9):2447-51. doi: 10.1073/pnas.80.9.2447. Proc Natl Acad Sci U S A. 1983. PMID: 6302683 Free PMC article.
-
DNA supercoiling and its effects on the structure of DNA.J Cell Sci Suppl. 1984;1:21-9. doi: 10.1242/jcs.1984.supplement_1.2. J Cell Sci Suppl. 1984. PMID: 6397472 Review.
-
DNA supercoiling in vivo.Biophys Chem. 1988 Feb;29(1-2):7-15. doi: 10.1016/0301-4622(88)87020-0. Biophys Chem. 1988. PMID: 2833949 Review.
Cited by
-
The human PTGR1 gene expression is controlled by TE-derived Z-DNA forming sequence cooperating with miR-6867-5p.Sci Rep. 2024 Feb 27;14(1):4723. doi: 10.1038/s41598-024-55332-x. Sci Rep. 2024. PMID: 38413664 Free PMC article.
-
Crystal structure of Z-DNA without an alternating purine-pyrimidine sequence.Proc Natl Acad Sci U S A. 1985 Jun;82(11):3611-5. doi: 10.1073/pnas.82.11.3611. Proc Natl Acad Sci U S A. 1985. PMID: 3858839 Free PMC article.
-
Direct evidence for the presence of left-handed conformation in a supramolecular assembly of polynucleotides.Nucleic Acids Res. 1989 May 25;17(10):3795-810. doi: 10.1093/nar/17.10.3795. Nucleic Acids Res. 1989. PMID: 2660102 Free PMC article.
-
Evidence for a new Z-type left-handed DNA helix: properties of Z(WC)-DNA.Nucleic Acids Res. 1990 Oct 25;18(20):6119-26. doi: 10.1093/nar/18.20.6119. Nucleic Acids Res. 1990. PMID: 2235494 Free PMC article.
-
ADAR1 masks the cancer immunotherapeutic promise of ZBP1-driven necroptosis.Nature. 2022 Jun;606(7914):594-602. doi: 10.1038/s41586-022-04753-7. Epub 2022 May 25. Nature. 2022. PMID: 35614224 Free PMC article.
References
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
