Nucleotide sequence analysis of the long terminal repeat of murine virus-like DNA (VL30) and its adjacent sequences: resemblance to retrovirus proviruses
- PMID: 6620466
- PMCID: PMC255309
- DOI: 10.1128/JVI.47.3.656-659.1983
Nucleotide sequence analysis of the long terminal repeat of murine virus-like DNA (VL30) and its adjacent sequences: resemblance to retrovirus proviruses
Abstract
VL30 DNA represents a retrovirus-like multigene family of mice whose genetic origin is unknown. We have now determined the primary nucleotide sequences and the adjacent sequences of the long terminal direct repeats (LTRs) possessed by a randomly selected VL30 unit. The LTR of the VL30 unit comprised 435 nucleotide base pairs and had an inverted repeat of five bases at its 5' and 3' termini. At the joints with flanking mouse DNA was the VL30 sequence (5')TG . . . CA(3') and a tetranucleotide direct repeat of flanking sequences. At the inner boundary of the 5' LTR was an 18-base sequence that is complementary to tRNApro, and at the inner boundary of the 3' LTR was a purine-rich tract ending with AATG. These results suggested that VL30 DNA used the same integration strategy that is exercised by retrovirus proviruses and transposable elements and that the VL30 LTR is synthesized in a similar way that the LTR of retroviruses is synthesized. The data thus reinforce the retrovirus-like nature of VL30 genetic information.
Similar articles
-
'Solo' large terminal repeats (LTR) of an endogenous retrovirus-like gene family (VL30) in the mouse genome.Nucleic Acids Res. 1984 Mar 12;12(5):2273-82. doi: 10.1093/nar/12.5.2273. Nucleic Acids Res. 1984. PMID: 6324110 Free PMC article.
-
Diverse long terminal repeats are associated with murine retroviruslike (VL30) elements.Mol Cell Biol. 1986 Apr;6(4):1276-82. doi: 10.1128/mcb.6.4.1276-1282.1986. Mol Cell Biol. 1986. PMID: 3023879 Free PMC article.
-
Terminal direct repeats in a retrovirus-like repeated mouse gene family.Nature. 1981 Jan 1;289(5793):83-5. doi: 10.1038/289083a0. Nature. 1981. PMID: 6256651
-
Molecular biology of type A endogenous retrovirus.Kitasato Arch Exp Med. 1990 Sep;63(2-3):77-90. Kitasato Arch Exp Med. 1990. PMID: 1710682 Review.
-
From a retrovirus infection of mice to a long noncoding RNA that induces proto-oncogene transcription and oncogenesis via an epigenetic transcription switch.Signal Transduct Target Ther. 2016 May 13;1:16007. doi: 10.1038/sigtrans.2016.7. eCollection 2016. Signal Transduct Target Ther. 2016. PMID: 29263895 Free PMC article. Review.
Cited by
-
Identification of an internal ribosome entry segment in the 5' region of the mouse VL30 retrotransposon and its use in the development of retroviral vectors.J Virol. 1999 Oct;73(10):8393-402. doi: 10.1128/JVI.73.10.8393-8402.1999. J Virol. 1999. PMID: 10482590 Free PMC article.
-
A single point mutation in the envelope gene is responsible for replication and XC fusion deficiency of the endogenous ecotropic C3H/He murine leukemia virus and for its repair in culture.J Virol. 1988 Mar;62(3):932-43. doi: 10.1128/JVI.62.3.932-943.1988. J Virol. 1988. PMID: 2828688 Free PMC article.
-
Isolation of cellular genes differentially expressed in mouse NIH 3T3 cells and a simian virus 40-transformed derivative: growth-specific expression of VL30 genes.Mol Cell Biol. 1985 Oct;5(10):2590-8. doi: 10.1128/mcb.5.10.2590-2598.1985. Mol Cell Biol. 1985. PMID: 3016508 Free PMC article.
-
Identification of a retrovirus-like repetitive element in human DNA.Proc Natl Acad Sci U S A. 1984 Dec;81(23):7510-4. doi: 10.1073/pnas.81.23.7510. Proc Natl Acad Sci U S A. 1984. PMID: 6095301 Free PMC article.
-
Primer binding sites corresponding to several tRNA species are present in DNAs of different members of the same retrovirus-like gene family (VL30).J Virol. 1985 Apr;54(1):236-9. doi: 10.1128/JVI.54.1.236-239.1985. J Virol. 1985. PMID: 3973981 Free PMC article.
References
Publication types
MeSH terms
Substances
Associated data
- Actions
- Actions
LinkOut - more resources
Full Text Sources
Medical
Miscellaneous
