doi: 10.1016/0014-5793(83)81070-9.
Fate of newly synthesized histones in G1 and G0 cells
- PMID: 6617889
- DOI: 10.1016/0014-5793(83)81070-9
Item in Clipboard
Fate of newly synthesized histones in G1 and G0 cells
FEBS Lett.
.
Free article
Abstract
We have shown that quiescent cells as well as those in the G1 phase of the cell cycle synthesize histones at a reduced but significant rate. Now, we show that the histones synthesized during G0 and G1 are stably incorporated into nuclei soon after synthesis. Micrococcal nuclease digestion of nuclei isolated from cells in G0 and G1 revealed that the specific histone variants synthesized in these different physiological states are found associated with DNA as nucleosomes. Nucleosomes were separated by polyacrylamide gel electrophoresis in a reducing buffer so that histone spot morphology, particularly that of the H3s was improved.
Similar articles
-
Patterns of histone variant synthesis can distinguish G0 from G1 cells.Cell. 1982 Dec;31(2 Pt 1):367-74. doi: 10.1016/0092-8674(82)90130-1. Cell. 1982. PMID: 7159927
-
G1 and S phase mammalian cells synthesize histones at equivalent rates.Cell. 1980 Aug;21(1):195-204. doi: 10.1016/0092-8674(80)90127-0. Cell. 1980. PMID: 7407911
-
Histone synthesis and deposition in the G1 and S phases of hepatoma tissue culture cells.Biochemistry. 1985 Nov 19;24(24):6921-30. doi: 10.1021/bi00345a026. Biochemistry. 1985. PMID: 3935167
-
Histone synthesis by lymphocytes in G0 and G1.Biochemistry. 1983 Apr 12;22(8):1778-83. doi: 10.1021/bi00277a006. Biochemistry. 1983. PMID: 6849885
-
Separation of basal histone synthesis from S-phase histone synthesis in dividing cells.Cell. 1981 Dec;27(2 Pt 1):321-30. doi: 10.1016/0092-8674(81)90415-3. Cell. 1981. PMID: 7199388 No abstract available.
Cited by
-
cAMP/phorbol ester response element is involved in transcriptional regulation of the human replacement histone gene H3.3B.Biochem J. 1998 Feb 1;329 ( Pt 3)(Pt 3):609-13. doi: 10.1042/bj3290609. Biochem J. 1998. PMID: 9445389 Free PMC article.
-
Nucleosomal histones of transcriptionally active/competent chromatin preferentially exchange with newly synthesized histones in quiescent chicken erythrocytes.Biochem J. 1990 Oct 1;271(1):67-73. doi: 10.1042/bj2710067. Biochem J. 1990. PMID: 2171504 Free PMC article.
-
Interrelationships of protein and DNA syntheses during replication of mammalian cells.Mol Cell Biol. 1985 Jun;5(6):1279-86. doi: 10.1128/mcb.5.6.1279-1286.1985. Mol Cell Biol. 1985. PMID: 4033653 Free PMC article.
-
Canonical and Variant Forms of Histone H3 Are Deposited onto the Human Cytomegalovirus Genome during Lytic and Latent Infections.J Virol. 2016 Oct 28;90(22):10309-10320. doi: 10.1128/JVI.01220-16. Print 2016 Nov 15. J Virol. 2016. PMID: 27605676 Free PMC article.
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
