Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1984 Jul;99(1 Pt 1):37-41.
doi: 10.1083/jcb.99.1.37.

Colchicine-binding activity distinguishes sea urchin egg and outer doublet tubulins

L Wilson, H P Miller, T A Pfeffer, K F Sullivan, H W Detrich 3rd

Colchicine-binding activity distinguishes sea urchin egg and outer doublet tubulins

L Wilson et al. J Cell Biol. 1984 Jul.

Abstract

The colchicine-binding activity of tubulin has been utilized to distinguish the tubulins from two distinct microtubule systems of the same species, the sea urchin Strongylocentrotus purpuratus. We have analyzed the colchicine-binding affinities of highly purified tubulins from the unfertilized eggs and from the flagellar outer doublet microtubules by van't Hoff analysis, and have found significant differences in the free energy, enthalpy, and entropy changes characterizing the binding of colchicine to the two tubulins. The data indicate that significant chemical differences in the tubulins from the two functionally distinct microtubule systems exist, and that the differences are expressed in the native forms of the tubulins. Our findings are discussed in terms of the possibility that the colchicine-binding site may be an important regulatory site on the tubulin molecule.

PubMed Disclaimer

Similar articles

See all similar articles

References

    1. Biochemistry. 1981 Apr 14;20(8):2316-24 - PubMed
    1. J Biol Chem. 1983 Apr 10;258(7):4190-4 - PubMed
    1. Nature. 1978 Nov 23;276(5686):411-3 - PubMed
    1. Exp Cell Res. 1973 Oct;81(2):285-92 - PubMed
    1. J Cell Biol. 1972 Nov;55(2):355-67 - PubMed

Publication types