Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Review
. 1983 Nov;97(5 Pt 1):1327-37.
doi: 10.1083/jcb.97.5.1327.

Regulation of chloroplast membrane function: protein phosphorylation changes the spatial organization of membrane components

L A Staehelin, C J Arntzen
Review

Regulation of chloroplast membrane function: protein phosphorylation changes the spatial organization of membrane components

L A Staehelin et al. J Cell Biol. 1983 Nov.

Abstract

A chlorophyll-protein complex of chloroplast membranes, which simultaneously serves as light-harvesting antenna and membrane adhesion factor, undergoes reversible, lateral diffusion between appressed and nonappressed membrane regions under the control of a protein kinase. The phosphorylation-dependent migration process regulates the amount of light energy that is delivered to the reaction centers of photosystems I and II (PS I and PS II), and thereby regulates their rate of turnover. This regulatory mechanism provides a rationale for the finding that the two photosystems are physically separated in chloroplast membranes (PS II in appressed, grana membranes, and PS I in nonappressed, stroma membranes). The feedback system involves the following steps: a membrane-bound kinase senses the rate of PS II vs. PS I turnover via the oxidation-reduction state of the plastoquinone pool, which shuttles electrons from PS II via cytochrome f to PS I. If activated, the kinase adds negative charge (phosphate) to a grana-localized pigment-protein complex. The change in its surface charge at a site critical for promoting membrane adhesion results in increased electrostatic repulsion between the membranes, unstacking, the lateral movement of the complex to adjacent stroma membranes, which differ in their functional composition. The general significance of this type of membrane regulatory mechanism is discussed.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Biochim Biophys Acta. 1981 Mar 12;635(1):53-62 - PubMed
    1. Biochemistry. 1977 Jun 14;16(12):2633-9 - PubMed
    1. Eur J Biochem. 1980 Sep;110(2):521-6 - PubMed
    1. J Cell Biol. 1980 Dec;87(3 Pt 1):728-35 - PubMed
    1. Arch Biochem Biophys. 1980 Apr 15;201(1):347-55 - PubMed

Publication types

MeSH terms

Substances