Nuclear-cytoplasmic transport and VAI RNA-independent translation of influenza viral messenger RNAs in late adenovirus-infected cells
- PMID: 6327069
- DOI: 10.1016/0092-8674(84)90378-7
Nuclear-cytoplasmic transport and VAI RNA-independent translation of influenza viral messenger RNAs in late adenovirus-infected cells
Abstract
Influenza virus-specific proteins are synthesized at essentially the same levels in late adenovirus-infected HeLa cells as in cells not infected with adenovirus, indicating that influenza viral mRNA escapes the blocks exerted by adenovirus against host-cell mRNA expression-transport from the nucleus and translation. A significant proportion of the influenza viral mRNAs synthesized in these cells possesses the 5' ends of the major late adenovirus transcripts, resulting from capped RNA-primed initiation of influenza viral mRNA synthesis. We determined whether the ability of influenza viral mRNA to escape from the adenovirus-imposed blocks is due to utilization of the adenovirus-specific transport and translational systems because of the adenovirus 5' ends on the influenza viral mRNAs, or to establishment of influenza virus-specific transport and translational systems. Our results indicate that the second mechanism is operating, as the transport of influenza viral mRNAs from the nucleus and their translation is independent of the presence of adenovirus 5' ends. Furthermore, efficient translation of influenza viral mRNAs, but not of either adenovirus or host mRNAs, occurs in cells infected by the adenovirus deletion mutant dl331 , which does not synthesize VAI RNA ( Thimmappaya et al., 1982). Consequently, utilizing the translational machinery that had been inactivated by adenovirus, influenza virus establishes a system that selectively translates influenza viral mRNAs.
Similar articles
-
Cellular mRNA translation is blocked at both initiation and elongation after infection by influenza virus or adenovirus.J Virol. 1986 Dec;60(3):1027-39. doi: 10.1128/JVI.60.3.1027-1039.1986. J Virol. 1986. PMID: 3023655 Free PMC article.
-
Adenovirus VAI RNA facilitates the initiation of translation in virus-infected cells.Cell. 1984 May;37(1):291-8. doi: 10.1016/0092-8674(84)90325-8. Cell. 1984. PMID: 6722874
-
The adenovirus L4 100-kilodalton protein is necessary for efficient translation of viral late mRNA species.J Virol. 1990 Jun;64(6):2732-42. doi: 10.1128/JVI.64.6.2732-2742.1990. J Virol. 1990. PMID: 2335816 Free PMC article.
-
Release of viruses and viral DNA from nucleus to cytoplasm of HeLa cells at late stages of productive adenovirus infection as revealed by electron microscope in situ hybridization.Biol Cell. 1998 Jan;90(1):5-38. doi: 10.1016/s0248-4900(98)80230-x. Biol Cell. 1998. PMID: 9691424 Review.
-
Translational control in influenza virus-infected cells.Enzyme. 1990;44(1-4):265-77. doi: 10.1159/000468764. Enzyme. 1990. PMID: 2133654 Review.
Cited by
-
Influenza virus mRNA translation revisited: is the eIF4E cap-binding factor required for viral mRNA translation?J Virol. 2007 Nov;81(22):12427-38. doi: 10.1128/JVI.01105-07. Epub 2007 Sep 12. J Virol. 2007. PMID: 17855553 Free PMC article.
-
Modification of eukaryotic initiation factor 4F during infection by influenza virus.J Virol. 1993 Jun;67(6):3027-35. doi: 10.1128/JVI.67.6.3027-3035.1993. J Virol. 1993. PMID: 8098776 Free PMC article.
-
How does influenza virus regulate gene expression at the level of mRNA translation? Let us count the ways.Gene Expr. 1993;3(2):109-18. Gene Expr. 1993. PMID: 8268717 Free PMC article. Review. No abstract available.
-
Regulation of protein synthesis in virus-infected animal cells.Adv Virus Res. 1986;31:229-92. doi: 10.1016/s0065-3527(08)60265-1. Adv Virus Res. 1986. PMID: 3019107 Free PMC article. Review.
-
The N-terminal half of the influenza virus NS1 protein is sufficient for nuclear retention of mRNA and enhancement of viral mRNA translation.Nucleic Acids Res. 1997 Nov 1;25(21):4271-7. doi: 10.1093/nar/25.21.4271. Nucleic Acids Res. 1997. PMID: 9336457 Free PMC article.
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources
