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. 1984 May;50(2):335-42.
doi: 10.1128/JVI.50.2.335-342.1984.

Expression of hepatitis B antigens with a simian virus 40 vector

H Will, R Cattaneo, E Pfaff, C Kuhn, M Roggendorf, H Schaller

Expression of hepatitis B antigens with a simian virus 40 vector

H Will et al. J Virol. 1984 May.

Abstract

Recombinant DNA molecules consisting of the simian virus 40 (SV40) early region and different subgenomic hepatitis B virus DNA fragments were constructed in vitro and packaged in vivo into SV40 capsids by using a complementing SV40 helper virus. Upon infection with these virus stocks the three known hepatitis B-specific antigens were expressed under SV40 control. The surface antigen was released into the medium, and the core antigen and its derivative hepatitis B e antigen were only detected intracellularly. Size analysis of the core gene product(s) by immunoblotting revealed the presence of a single protein species identical with the 21-kilodalton core antigen isolated from human liver. The hepatitis B core antigen expressing construct did not contain a putative precore sequence, indicating that such a sequence is not needed for hepatitis B core antigen synthesis in animal cells. S1 analysis demonstrated the use of SV40 signals for initiation and polyadenylation of the core gene transcripts. In addition, a processing-polyadenylation signal was identified within the core gene.

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References

    1. EMBO J. 1983;2(1):21-5 - PubMed
    1. Proc Natl Acad Sci U S A. 1969 Apr;62(4):1159-66 - PubMed
    1. Nature. 1970 Aug 15;227(5259):680-5 - PubMed
    1. J Mol Biol. 1975 Jan 5;91(1):121-3 - PubMed
    1. J Mol Biol. 1975 Nov 5;98(3):503-17 - PubMed

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