Comparative Study
doi: 10.1016/0378-1119(83)90039-2.
New versatile cloning and sequencing vectors based on bacteriophage M13
- PMID: 6323254
- DOI: 10.1016/0378-1119(83)90039-2
Item in Clipboard
Comparative Study
New versatile cloning and sequencing vectors based on bacteriophage M13
Gene.
1983 Dec.
Abstract
A new pair of cloning and sequencing vectors based on bacteriophage M13mp7 has been developed. These vectors (M13tg130 and M13tg131) contain, in addition to the EcoRI, BamHI, HindIII, SmaI, SalI and PstI sites present in other vectors [cf., M13mp8 and M13mp9, Messing and Vieira, Gene 19 (1982) 269-276], unique restriction recognition sequences for the enzymes EcoRV, KpnI, SphI, SstI and XbaI. A restriction site for the enzyme BglII has been incorporated into the polylinker region of one of the vector pair to permit rapid discrimination between the two vectors.
Similar articles
-
A new selective phage cloning vector, lambda 2001, with sites for XbaI, BamHI, HindIII, EcoRI, SstI and XhoI.Gene. 1984 Dec;32(1-2):217-24. doi: 10.1016/0378-1119(84)90049-0. Gene. 1984. PMID: 6099311
-
Construction and characterization of new coliphage M13 cloning vectors.Gene. 1980 Nov;11(3-4):207-18. doi: 10.1016/0378-1119(80)90061-x. Gene. 1980. PMID: 6260570
-
Restriction maps for twenty-one Charon vector phages.J Virol. 1980 Jan;33(1):401-10. doi: 10.1128/JVI.33.1.401-410.1980. J Virol. 1980. PMID: 6245241 Free PMC article.
-
[A simple system of cloning in phage M13 and DNA sequencing with terminators].Mol Biol (Mosk). 1988 Sep-Oct;22(5):1164-97. Mol Biol (Mosk). 1988. PMID: 3065614 Review. Russian.
-
Cloning in M13 phage or how to use biology at its best.Gene. 1991 Apr;100:3-12. doi: 10.1016/0378-1119(91)90344-b. Gene. 1991. PMID: 2055478 Review.
Cited by
-
Four plasmid genes are required for colicin V synthesis, export, and immunity.J Bacteriol. 1987 Jun;169(6):2466-70. doi: 10.1128/jb.169.6.2466-2470.1987. J Bacteriol. 1987. PMID: 3034857 Free PMC article.
-
Transcriptional termination at a fully rho-independent site in Escherichia coli is prevented by uninterrupted translation of the nascent RNA.EMBO J. 1987 Apr;6(4):1115-9. doi: 10.1002/j.1460-2075.1987.tb04866.x. EMBO J. 1987. PMID: 3036492 Free PMC article.
-
Trans-acting transposase mutant from Tn5.Proc Natl Acad Sci U S A. 1991 Jul 15;88(14):6072-6. doi: 10.1073/pnas.88.14.6072. Proc Natl Acad Sci U S A. 1991. PMID: 1648727 Free PMC article.
-
Conjugative mobilization as an alternative vector delivery system for lactic streptococci.Appl Environ Microbiol. 1987 Oct;53(10):2405-13. doi: 10.1128/aem.53.10.2405-2413.1987. Appl Environ Microbiol. 1987. PMID: 3122654 Free PMC article.
-
The plastid rpoA gene encoding a protein homologous to the bacterial RNA polymerase alpha subunit is expressed in pea chloroplasts.Mol Gen Genet. 1989 May;217(1):77-84. doi: 10.1007/BF00330945. Mol Gen Genet. 1989. PMID: 2671652
Publication types
MeSH terms
Substances
Associated data
- Actions
- Actions
- Actions
- Actions
LinkOut - more resources
Full Text Sources
Other Literature Sources
