Import of proteins into mitochondria: a 70 kilodalton outer membrane protein with a large carboxy-terminal deletion is still transported to the outer membrane
- PMID: 6321150
- PMCID: PMC555428
- DOI: 10.1002/j.1460-2075.1983.tb01717.x
Import of proteins into mitochondria: a 70 kilodalton outer membrane protein with a large carboxy-terminal deletion is still transported to the outer membrane
Abstract
The yeast mitochondrial outer membrane contains a major 70-kd protein which is coded by a nuclear gene. Two forms of this gene were isolated from a yeast genomic clone bank: the intact gene, and a truncated gene which had lost a large part of its 3' end during the cloning procedure. Upon transformation into yeast, both the intact and the truncated gene are expressed; the truncated gene generates a shortened protein missing 203 amino acids from the carboxy-terminus. This truncated polypeptide reacts with a monoclonal antibody against the authentic 70-kd protein and is transported to the mitochondrial outer membrane. By integrative transformation, we have constructed a yeast mutant which lacks the 70-kd protein and is unable to adapt to growth on a nonfermentable carbon source at 37 degrees C. This phenotypic lesion can be corrected by transforming the mutant with the intact, but not the truncated gene. The carboxy-terminal sequence of 203 amino acids is thus necessary for the function of the protein, but not for its targeting to the mitochondrion.
Similar articles
-
Import of proteins into mitochondria: nucleotide sequence of the gene for a 70-kd protein of the yeast mitochondrial outer membrane.EMBO J. 1983;2(12):2169-72. doi: 10.1002/j.1460-2075.1983.tb01718.x. EMBO J. 1983. PMID: 6365533 Free PMC article.
-
The major 45-kDa protein of the yeast mitochondrial outer membrane is not essential for cell growth or mitochondrial function.J Biol Chem. 1989 Dec 15;264(35):21091-6. J Biol Chem. 1989. PMID: 2687271
-
Isolation and characterization of the gene for a yeast mitochondrial import receptor.Nature. 1990 Oct 4;347(6292):488-91. doi: 10.1038/347488a0. Nature. 1990. PMID: 2170848
-
A 70-kd protein of the yeast mitochondrial outer membrane is targeted and anchored via its extreme amino terminus.EMBO J. 1984 Dec 20;3(13):3157-64. doi: 10.1002/j.1460-2075.1984.tb02274.x. EMBO J. 1984. PMID: 6396085 Free PMC article.
-
Molecular cloning and sequencing of cDNA for yeast porin, an outer mitochondrial membrane protein: a search for targeting signal in the primary structure.EMBO J. 1985 Mar;4(3):769-74. doi: 10.1002/j.1460-2075.1985.tb03695.x. EMBO J. 1985. PMID: 2408884 Free PMC article.
Cited by
-
A deletion that includes the signal peptidase cleavage site impairs processing, glycosylation, and secretion of cell surface yeast acid phosphatase.Mol Cell Biol. 1984 Dec;4(12):2668-75. doi: 10.1128/mcb.4.12.2668-2675.1984. Mol Cell Biol. 1984. PMID: 6098819 Free PMC article.
-
Porin 1 Modulates Autophagy in Yeast.Cells. 2021 Sep 14;10(9):2416. doi: 10.3390/cells10092416. Cells. 2021. PMID: 34572064 Free PMC article.
-
PET genes of Saccharomyces cerevisiae.Microbiol Rev. 1990 Sep;54(3):211-25. doi: 10.1128/mr.54.3.211-225.1990. Microbiol Rev. 1990. PMID: 2215420 Free PMC article. Review.
-
Subunit IV of yeast cytochrome c oxidase: cloning and nucleotide sequencing of the gene and partial amino acid sequencing of the mature protein.EMBO J. 1984 Dec 1;3(12):2831-7. doi: 10.1002/j.1460-2075.1984.tb02216.x. EMBO J. 1984. PMID: 6098449 Free PMC article.
-
The END3 gene encodes a protein that is required for the internalization step of endocytosis and for actin cytoskeleton organization in yeast.Mol Biol Cell. 1994 Sep;5(9):1023-37. doi: 10.1091/mbc.5.9.1023. Mol Biol Cell. 1994. PMID: 7841519 Free PMC article.
References
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases
