A colorimetric assay for the measurement of the sensitivity of herpes simplex viruses to antiviral agents
- PMID: 6320716
- DOI: 10.1016/0166-3542(83)90001-3
A colorimetric assay for the measurement of the sensitivity of herpes simplex viruses to antiviral agents
Abstract
A quantitative colorimetric method for measuring the inhibition of viral cytopathic effects has been adapted to the assay of antiviral compounds. Drug-treated, virus-infected cultures in microtiter plates were stained with the vital dye neutral red and the amount of dye incorporated was determined in a multichannel spectrophotometer. The technique required smaller volumes of reagents, was more easily automated than the standard plaque reduction assay and had good reproducibility. Standard conditions of 30 infectious units of challenge virus and 72-h incubation were judged to be optimal. Median inhibitory concentrations (ID50) for a number of compounds were approximately tenfold higher in the dye-uptake assay compared with the plaque reduction assay, possibly related to the higher multiplicity of infection required to give the desired level of cytopathic effect in the microtiter method.
Similar articles
-
A rapid and automated colorimetric assay for evaluating the sensitivity of herpes simplex strains to antiviral drugs.J Biol Stand. 1986 Jul;14(3):201-11. doi: 10.1016/0092-1157(86)90004-1. J Biol Stand. 1986. PMID: 3020058
-
Methods for screening and profiling inhibitors of herpes simplex viruses.Methods Mol Biol. 2013;1030:303-13. doi: 10.1007/978-1-62703-484-5_23. Methods Mol Biol. 2013. PMID: 23821277
-
A microtiter virus yield reduction assay for the evaluation of antiviral compounds against human cytomegalovirus and herpes simplex virus.J Virol Methods. 1990 Apr;28(1):101-6. doi: 10.1016/0166-0934(90)90091-s. J Virol Methods. 1990. PMID: 2161417
-
Virus drug-resistance: mechanisms and consequences.Antiviral Res. 1984 Apr;4(1-2):1-42. doi: 10.1016/0166-3542(84)90023-8. Antiviral Res. 1984. PMID: 6331303 Review. No abstract available.
-
Virus-drug resistance: thymidine kinase-deficient (TK-) mutants of herpes simplex virus. Therapeutic approaches.Ann Ist Super Sanita. 1987;23(4):841-7. Ann Ist Super Sanita. 1987. PMID: 2837935 Review. No abstract available.
Cited by
-
Drug resistance patterns of herpes simplex virus isolates from patients treated with acyclovir.Antimicrob Agents Chemother. 1985 Dec;28(6):740-4. doi: 10.1128/AAC.28.6.740. Antimicrob Agents Chemother. 1985. PMID: 3002245 Free PMC article.
-
Ostreid herpesvirus type 1 replication and host response in adult Pacific oysters, Crassostrea gigas.Vet Res. 2014 Oct 8;45(1):103. doi: 10.1186/s13567-014-0103-x. Vet Res. 2014. PMID: 25294338 Free PMC article.
-
Cell-based Assays to Identify Inhibitors of Viral Disease.Expert Opin Drug Discov. 2008 Jun 1;3(6):671-676. doi: 10.1517/17460441.3.6.671. Expert Opin Drug Discov. 2008. PMID: 19750206 Free PMC article.
-
Antiviral Activity of Zinc Oxide Nanoparticles Mediated by Plumbago indica L. Extract Against Herpes Simplex Virus Type 1 (HSV-1).Int J Nanomedicine. 2021 Dec 18;16:8221-8233. doi: 10.2147/IJN.S339404. eCollection 2021. Int J Nanomedicine. 2021. PMID: 34955639 Free PMC article.
-
Environmentally Friendly Valorization of Solieria filiformis (Gigartinales, Rhodophyta) from IMTA Using a Biorefinery Concept.Mar Drugs. 2018 Dec 6;16(12):487. doi: 10.3390/md16120487. Mar Drugs. 2018. PMID: 30563236 Free PMC article.
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
