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. 1983 Dec;18(6):1329-34.
doi: 10.1128/jcm.18.6.1329-1334.1983.

Biotin-avidin-amplified enzyme immunoassay for detection of herpes simplex virus antigen in clinical specimens

K Adler-Storthz, C Kendall, R C Kennedy, R D Henkel, G R Dreesman

Biotin-avidin-amplified enzyme immunoassay for detection of herpes simplex virus antigen in clinical specimens

K Adler-Storthz et al. J Clin Microbiol. 1983 Dec.

Abstract

A biotin-avidin-amplified enzyme-linked immunosorbent assay (B-A ELISA) has been developed to detect herpes simplex virus type 1 (HSV-1) and HSV-2 antigens in clinical specimens. The test was designed as a solid-phase, double-antibody, sandwich assay in which plates were coated with a polyclonal rabbit immunoglobulin G anti-HSV reagent, and the sandwich antibody was a biotin-labeled mouse immunoglobulin M monoclonal antibody that reacts with a common antigen associated with HSV-1 and HSV-2. The test can be completed in 4 h if antibody-coated plates are available. The detection limit of the B-A ELISA, determined by titration of virus stocks, was found to be approximately 90 PFU or 6 X 10(3) physical particles of either HSV-1 or HSV-2 per 50 microliter of virus stock. The following results were obtained in a study in which swabs were taken from a variety of lesions and assayed for infectivity in tissue culture and by B-A ELISA. Of 421 suspected HSV lesions tested, 69 were positive by both tests and 159 were negative by both tests. A total of 122 were positive by B-A ELISA but negative for infectivity. Seventy-one were negative by B-A ELISA but contained infectious virus. The HSV specificity of the assay was substantiated by partial blocking of reactivity with rabbit immunoglobulin G anti-HSV and by the absence of reactivity with a nonspecific biotin-labeled mouse immunoglobulin M monoclonal antibody.

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References

    1. J Bacteriol. 1966 Feb;91(2):803-12 - PubMed
    1. J Immunol. 1967 Dec;99(6):1106-14 - PubMed
    1. J Gen Virol. 1969 Apr;4(3):321-8 - PubMed
    1. Adv Protein Chem. 1975;29:85-133 - PubMed
    1. J Histochem Cytochem. 1979 Aug;27(8):1131-9 - PubMed

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