Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1983 Sep;47(3):600-10.
doi: 10.1128/JVI.47.3.600-610.1983.

Polyomavirus large T antigen binds independently to multiple, unique regions on the viral genome

B J Pomerantz, C R Mueller, J A Hassell

Polyomavirus large T antigen binds independently to multiple, unique regions on the viral genome

B J Pomerantz et al. J Virol. 1983 Sep.

Abstract

To map the polyomavirus large T antigen binding sites on the viral genome we employed a quantitative immunoassay. Defined, radiolabeled fragments of the viral genome were reacted with crude nuclear extracts prepared from lytically infected mouse 3T6 cells, and the fragments bound by large T antigen were immunoprecipitated with anti-T serum and Formalin-fixed Staphylococcus aureus. The immunoprecipitated DNA was then analyzed by gel electrophoresis and autoradiography. By the use of a variety of restriction endonuclease-generated fragments of wild-type and mutant viral DNAs, the region of high-affinity binding was localized to a 153-base-pair stretch between nucleotides 5292 and 152. At least two independent binding sites lie within this region, one upstream and the other downstream of the Bg/I site at nucleotide 87. One of the binding sites is located within sequences required in cis for DNA replication; the other overlaps the TATA box and cap sites of the early transcription unit. The two sites share a common sequence, A/TGAGGC-N4/5-A/TGAGGC, which may serve as the recognition sequence for large T antigen.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Virology. 1973 Sep;55(1):127-35 - PubMed
    1. Science. 1974 Mar 1;183(4127):810-6 - PubMed
    1. J Gen Virol. 1974 Oct;25(1):133-42 - PubMed
    1. Proc Natl Acad Sci U S A. 1977 Oct;74(10):4666-70 - PubMed
    1. Cell. 1978 Jan;13(1):165-79 - PubMed

Publication types

LinkOut - more resources