doi: 10.1007/BF00330903.
Plasmids expressing the Herpes simplex virus thymidine kinase gene in mammalian and bacterial cells
- PMID: 6310348
- DOI: 10.1007/BF00330903
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Plasmids expressing the Herpes simplex virus thymidine kinase gene in mammalian and bacterial cells
Mol Gen Genet.
1983.
Abstract
Two plasmids containing either the complete thymidine kinase gene of Herpes simplex virus type I (pSK2) or the gene without the remote control sequence (pSK1) just behind the lac promoter and the first codons of the lacZ gene were constructed. Both plasmids efficiently transform mouse Ltk- cells as well as E. coli tk- cells to the Tk+ phenotype and are well suited for plasmid rescue from transformed mouse cells by direct functional selection for tk expression using a tk- mutant of E. coli C600.
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