Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Comparative Study
. 1983 Jul;80(14):4218-22.
doi: 10.1073/pnas.80.14.4218.

Structure and biological activity of v-raf, a unique oncogene transduced by a retrovirus

U R Rapp, M D Goldsborough, G E Mark, T I Bonner, J Groffen, F H Reynolds Jr, J R Stephenson
Comparative Study

Structure and biological activity of v-raf, a unique oncogene transduced by a retrovirus

U R Rapp et al. Proc Natl Acad Sci U S A. 1983 Jul.

Abstract

We have molecularly cloned a unique acutely transforming replication-defective mouse type C virus (3611-MSV) and characterized its acquired oncogene. The viral genome closely resembles Moloney (M) murine leukemia virus (MuLV), except for a substitution in M-MuLV in the middle of p30 and the middle of the polymerase gene (pol). Heteroduplex analysis revealed that 2.4 kilobases of M-MuLV DNA were replaced by 1.2 kilobases of cellular DNA. The junctions between viral and cellular sequences were determined by DNA sequence analysis to be 517 nucleotides into the p30 sequence and 1,920 nucleotides into the polymerase sequence. Comparison of the transforming gene from 3611-MSV, designated v-raf, with previously isolated retrovirus oncogenes either by direct hybridization or by comparison of restriction fragments of their cellular homologs shows it to be unique. Transfection of NIH 3T3 cells with cloned 3611-MSV proviral DNA leads to highly efficient transformation and the recovered virus elicits tumors in mice typical of the 3611-MSV virus. Transfected NIH 3T3 cells express two 3611-MSV-specific polyproteins (P75 and P90), both of which contain NH2-terminal gag gene-encoded components linked to the acquired sequence (v-raf) translational product. The cellular homolog, c-raf, is present in one or two copies per haploid genome in mouse and human DNA.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Virology. 1973 Apr;52(2):456-67 - PubMed
    1. Nature. 1976 Mar 11;260(5547):170-3 - PubMed
    1. Proc Natl Acad Sci U S A. 1977 Feb;74(2):789-92 - PubMed
    1. Science. 1977 Apr 8;196(4286):175-7 - PubMed
    1. Science. 1977 Apr 8;196(4286):180-2 - PubMed

Publication types