Introduction of superhelical turns into DNA by adenoviral core proteins and chromatin assembly factors
- PMID: 6304339
- PMCID: PMC256551
- DOI: 10.1128/JVI.46.3.749-755.1983
Introduction of superhelical turns into DNA by adenoviral core proteins and chromatin assembly factors
Abstract
The interaction in vitro between adenoviral histone-like proteins and DNA in the presence of chromatin assembly factors was investigated. Viral core protein VII or its precursor pVII was incubated with DNA in the presence of an extract of HeLa cell chromatin, which mediates nucleosome assembly from histones and DNA. We have demonstrated that either protein can introduce superhelical turns into relaxed closed-circular DNA and that the presence of chromatin extract is necessary for the supertwisting effect. A greater density of superhelical turns was produced by pVII than by VII, but neither protein-DNA interaction resulted in the "physiological" amount of supertwisting produced by histones. The inhibition of histone-induced supercoiling by both proteins and the protection of turns in supertwisted starting material are also described. The nucleosome assembly factor, nucleoplasmin, fails to mediate the introduction of superhelical turns by VII or pVII.
Similar articles
-
Folding of the DNA double helix in chromatin-like structures from simian virus 40.Proc Natl Acad Sci U S A. 1975 May;72(5):1843-7. doi: 10.1073/pnas.72.5.1843. Proc Natl Acad Sci U S A. 1975. PMID: 168578 Free PMC article.
-
Novobiocin inhibits passive chromatin assembly in vitro.EMBO J. 1986 Dec 1;5(12):3305-11. doi: 10.1002/j.1460-2075.1986.tb04644.x. EMBO J. 1986. PMID: 3028779 Free PMC article.
-
DNA supercoiling facilitates the assembly of transcriptionally active chromatin on the adenovirus major late promoter.Biochem Biophys Res Commun. 1992 Jul 31;186(2):963-9. doi: 10.1016/0006-291x(92)90840-h. Biochem Biophys Res Commun. 1992. PMID: 1323295
-
Chromatin replication, reconstitution and assembly.Mol Cell Biochem. 1983;55(2):99-112. doi: 10.1007/BF00673705. Mol Cell Biochem. 1983. PMID: 6355815 Review.
-
The structure of chromatin.Sci Prog. 1981 Autumn;67(267):399-410. Sci Prog. 1981. PMID: 6276974 Review. No abstract available.
Cited by
-
Adenovirus Core Proteins: Structure and Function.Viruses. 2021 Feb 28;13(3):388. doi: 10.3390/v13030388. Viruses. 2021. PMID: 33671079 Free PMC article. Review.
-
Activation of the adenovirus 2 protein IX promoter by DNA replication in a transient expression assay.Nucleic Acids Res. 1987 Mar 11;15(5):2235-50. doi: 10.1093/nar/15.5.2235. Nucleic Acids Res. 1987. PMID: 3031591 Free PMC article.
-
Human Adenovirus Gene Expression and Replication Is Regulated through Dynamic Changes in Nucleoprotein Structure throughout Infection.Viruses. 2023 Jan 5;15(1):161. doi: 10.3390/v15010161. Viruses. 2023. PMID: 36680201 Free PMC article. Review.
-
Adenovirus protein VII functions throughout early phase and interacts with cellular proteins SET and pp32.J Virol. 2005 Feb;79(4):2474-83. doi: 10.1128/JVI.79.4.2474-2483.2005. J Virol. 2005. PMID: 15681448 Free PMC article.
-
Chromatin structure of adenovirus DNA throughout infection.Nucleic Acids Res. 2012 Mar;40(6):2369-76. doi: 10.1093/nar/gkr1076. Epub 2011 Nov 23. Nucleic Acids Res. 2012. PMID: 22116065 Free PMC article. Review.
References
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
